Department of Agricultural Environment Protection, University of Agriculture in Krakow, Krakow, Poland.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Dec;44(14):1592-7. doi: 10.1080/10934520903263603.
The aim of the investigation was to assess the influence of commercial and carboxylated multi-walled carbon nanotubes (MWCNTs) on conidia of the entomopathogenic fungus Paecilomyces fumosoroseus. The commercial MWCNTs had an external diameter of about 40-60 nm, a length of 300-600 nm, a specific density of 140 to 300 g/dm(3) and a carbon content of above 80%. Carboxylated MWCNTs were obtained by oxidizing commercial MWCNT by heating in HNO(3), filtering, washing with water and drying. Conidia after different times of contact (from 1 to 865 hours) with nanomaterials in aqueous solutions were cultured for linear and biomass growth. Growth and sporification of mycelium after culture were evaluated. MWCNTs are not greatly cytotoxic for P. fumosoroseus conidia in the applied concentrations. The linear growth of mycelium obtained from conidia after contact with nanotubes was inhibited only in 2 (out of 18) cases (ones for both kinds of nanotubes). Carboxylated nanotubes did not inhibit biomass growth at all, but commercial nanotubes inhibited biomass growth in 2 cases. Sporification was the feature most strongly modified by carbon nanotubes. The commercial nanotubes again more strongly limited sporification of mycelium than carboxylated nanotubes did. The relatively greater influence of commercial versus carboxylated nanotubes was observed in the experiments.
本研究旨在评估商用和羧基化多壁碳纳米管（MWCNTs）对真菌玫烟色棒束孢分生孢子的影响。商用 MWCNTs 的外径约为 40-60nm，长度为 300-600nm，密度为 140-300g/dm(3)，碳含量高于 80%。羧基化 MWCNTs 通过在 HNO(3)中加热氧化商用 MWCNT 制得，经过过滤、水洗和干燥处理。将纳米材料在水溶液中与分生孢子接触不同时间（1-865 小时）后培养用于线性和生物量生长。培养后评估菌丝的生长和产孢情况。在所应用的浓度下，MWCNTs 对玫烟色棒束孢分生孢子的细胞毒性不大。接触纳米管后的分生孢子生成的菌丝线性生长仅在 2 种情况下（两种纳米管各 1 种）受到抑制。羧基化纳米管完全不抑制生物量生长，而商用纳米管在 2 种情况下抑制生物量生长。产孢是受碳纳米管影响最大的特征。商用纳米管再次比羧基化纳米管更强烈地限制了菌丝的产孢。在实验中观察到商用纳米管相对于羧基化纳米管具有更大的影响。
