School of Life Science, Huainan Normal University, Huainan 232001, China.
School of Life Science, Huainan Normal University, Huainan 232001, China.
J Hazard Mater. 2014 Jun 15;274:404-12. doi: 10.1016/j.jhazmat.2014.04.036. Epub 2014 Apr 25.
Increasing industrialization of multi-walled carbon nanotubes (MWCNTs) would inevitably lead to their release into the environment and combination with heavy metals. However, studies concerning the combined effects of MWCNTs and heavy metals on agricultural crops are limited. Herein, effects and mechanisms of carboxylated MWCNTs (MWCNTs-COOH) (2.5, 5 and 10mg/L) and their combination with 20 μM Pb and 5 μM Cd (shortened as Pb+Cd) on Vicia faba L. seedlings were investigated. The results showed that the MWCNTs-COOH disturbed the imbalance of nutrient elements, and caused oxidative stress and damages in the leaves. Additionally, the combination of MWCNTs-COOH with Pb+Cd resulted in enrichment of Pb and Cd, and deterioration of oxidative damages compared with the treatments of MWCNTs-COOH or Pb+Cd alone in the leaves. As the results, the concentrations of MWCNTs-COOH not only caused oxidative stress, but also exacerbated the biochemical and subcellular damages due to the treatment of Pb+Cd in the leaves. It also suggests that persistent release of MWCNTs-COOH into the environment may cause phytotoxicity and aggravate ecological risks due to combination of heavy metals.
随着多壁碳纳米管（MWCNTs）工业化程度的提高，它们不可避免地会释放到环境中并与重金属结合。然而，关于 MWCNTs 和重金属对农作物的联合作用的研究还很有限。在此，研究了羧基化多壁碳纳米管（MWCNTs-COOH）（2.5、5 和 10mg/L）及其与 20μM Pb 和 5μM Cd（简称 Pb+Cd）组合对蚕豆幼苗的影响和作用机制。结果表明，MWCNTs-COOH 扰乱了营养元素的平衡，导致叶片发生氧化应激和损伤。此外，与 MWCNTs-COOH 或 Pb+Cd 单独处理相比，MWCNTs-COOH 与 Pb+Cd 结合导致叶片中 Pb 和 Cd 的富集以及氧化损伤的恶化。因此，MWCNTs-COOH 的浓度不仅引起氧化应激，而且由于 Pb+Cd 的处理而加剧了叶片中的生化和亚细胞损伤。这也表明，MWCNTs-COOH 的持续释放到环境中可能由于重金属的结合而导致植物毒性并加重生态风险。
