核酸与吡虫啉的相互作用及其应用研究
Study on the interaction between nucleic acids and imidacloprid and its application.
作者信息
Hou Shicong, Jiang Xiaofeng, Du Fengpei, Cong Huifang, Li Ying, Jie Nianqin
机构信息
College of Science, China Agricultural University, Beijing, PR China.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2009 Nov;28(11):989-97. doi: 10.1080/15257770903362156.
The determination of imidacloprid with DNA via a resonance light scattering (RLS) technique was developed. The RLS of DNA was remarkably quenched after adding imidacloprid in aqueous medium of pH 2.10. An RLS peak at 311 nm was found, and the quenched intensity of RLS at this wavelength was proportional to the concentration of imidacloprid. The linear range of the calibration curve was approximately 0.02-2 microg/mL with the detection limit (S/N = 3) of 0.02 ng/mL. The imidacloprid in river water, cucumbers, and apple samples was determined. The recovery rates were in the range of 91.9% to 95.20%, 97.2% to 111.3%, and 94.5% to 114.8%, respectively. The mechanism of the reaction between imidacloprid and DNA is also discussed.
建立了通过共振光散射（RLS）技术用DNA测定吡虫啉的方法。在pH 2.10的水性介质中加入吡虫啉后，DNA的RLS显著猝灭。发现311 nm处有一个RLS峰，该波长下RLS的猝灭强度与吡虫啉浓度成正比。校准曲线的线性范围约为0.02 - 2 μg/mL，检测限（S/N = 3）为0.02 ng/mL。测定了河水、黄瓜和苹果样品中的吡虫啉。回收率分别在91.9%至95.20%、97.2%至111.3%和94.5%至114.8%范围内。还讨论了吡虫啉与DNA之间反应的机理。
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