Xiao Xi-lin, Wang Yong-sheng, Li Gui-rong, Lü Chang-yin
College of Public Health, Nanhua University, Hengyang 421001, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Feb;24(2):190-3.
Resonance light scattering (RLS) spectra of toluidine blue (TB) with DNA was studied. The RLS of TB was greatly enhanced by DNA in the range of pH 10-11. A RLS peak at 350 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-900 ng x mL(-1) with the detection limit of 6.75 ng x mL(-1) for the ctDNA, and for fsDNA the linear range was 0-900 ng x mL(-1) with the detection limit of 2.99 ng x mL(-1). Precision at 500 ng x mL(-1) for the two nucleic acids was 3.7% and 5.6%, respectively. Four synthetic samples were determined satisfactorily.
研究了甲苯胺蓝（TB）与DNA的共振光散射（RLS）光谱。在pH 10 - 11范围内，DNA能极大地增强TB的RLS。发现了一个350 nm处的RLS峰，该波长处RLS的增强强度与DNA浓度成正比。校准曲线的线性范围对于ctDNA为0 - 900 ng x mL(-1)，检测限为6.75 ng x mL(-1)；对于fsDNA，线性范围为0 - 900 ng x mL(-1)，检测限为2.99 ng x mL(-1)。两种核酸在500 ng x mL(-1)时的精密度分别为3.7%和5.6%。对四个合成样品进行了测定，结果令人满意。
