College of Agronomy, Shandong Agricultural University, Taian, Shandong, PR China.
J Sep Sci. 2010 Apr;33(8):1147-55. doi: 10.1002/jssc.200900726.
An optimized microwave-assisted extraction (MAE) method and an efficient HPLC analysis method were developed for fast extraction and simultaneous determination of oleanolic acid and ursolic acid in the fruit of Chaenomeles sinensis. The open vessel MAE process was optimized by using a central composite experimental design. The optimal conditions identified were microwave power 600 W, temperature 52 degrees C, solvent to material ratio 32 mL/g and extraction time 7 min. The results showed that MAE is a more rapid extraction method with higher yield and lower solvent consumption. The HPLC-photodiode array detection analysis method was validated to have good linearity, precision, reproduction and accuracy. Compared with conventional extraction and analysis methods, MAE-HPLC-photodiode array detection is a faster, convenient and appropriate method for determination of oleanolic acid and ursolic acid in the fruits of C. sinensis.
建立了一种优化的微波辅助提取（MAE）方法和高效的 HPLC 分析方法，用于快速提取和同时测定贴梗海棠果实中的齐墩果酸和熊果酸。采用中心复合实验设计对敞口 MAE 过程进行了优化。确定的最佳条件为：微波功率 600 W、温度 52℃、溶剂与物料比 32 mL/g 和提取时间 7 min。结果表明，MAE 是一种更快速的提取方法，具有更高的产率和更低的溶剂消耗。HPLC-光电二极管阵列检测分析方法具有良好的线性、精密度、重现性和准确性。与传统的提取和分析方法相比，MAE-HPLC-光电二极管阵列检测是一种更快、更方便、更合适的测定贴梗海棠果实中齐墩果酸和熊果酸的方法。
