State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, China.
J Sep Sci. 2013 Aug;36(16):2672-9. doi: 10.1002/jssc.201300317.
An optimized microwave-assisted extraction (MAE) method and RP-HPLC method were developed for the simultaneous extraction and determination of rutin, forsythiaside A, and phillyrin in the fruits of Forsythia suspensa. The key parameters of the open-vessel MAE process were optimized. A mixed solvent of methanol and water (70:30, v/v) was most suitable for the simultaneous extraction of the three components. The sample was soaked for 10 min before extraction. The optimized conditions were: microwave power 400 W, temperature 70°C, solvent-to-material ratio 30 mL/g, and extraction time 1 min. Compared to conventional extraction methods, the proposed method can simultaneously extract the three components in high yields and was proved to be a more rapid method with a lower solvent consumption. The optimized HPLC-photodiode array detection analysis was validated to have good linearity, precision, accuracy, and sensitivity. The developed MAE followed by RP-HPLC is a fast and appropriate method for the simultaneous extraction and determination of rutin, forsythiaside A, and phillyrin in the fruits of F. suspensa.
建立了一种优化的微波辅助提取（MAE）方法和反相高效液相色谱法（RP-HPLC），用于同时提取和测定连翘果实中的芦丁、连翘苷 A 和连翘酯苷 A。优化了敞口 MAE 过程的关键参数。甲醇和水（70:30，v/v）的混合溶剂最适合同时提取这三种成分。在提取前，样品浸泡 10 min。优化条件为：微波功率 400 W，温度 70°C，溶剂与物料比 30 mL/g，提取时间 1 min。与传统提取方法相比，该方法可以同时以高收率提取这三种成分，并且被证明是一种更快速、溶剂消耗更低的方法。优化的 HPLC-光电二极管阵列检测分析具有良好的线性、精密度、准确性和灵敏度。建立的 MAE 结合 RP-HPLC 是一种快速、合适的方法，可用于同时提取和测定连翘果实中的芦丁、连翘苷 A 和连翘酯苷 A。
