Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China.
Gynecol Endocrinol. 2010 Jul;26(7):549-53. doi: 10.3109/09513591003632290.
To evaluate the tissue damage of in vitro porcine ovarian drilling in saline solution mimicking transvaginal hydrolaparoscopy (THL).
In vitro ovarian drilling with different current and activation time was applied on fresh porcine ovaries, 15 ovaries in each group and 2 punctures in each ovary. Drilling using 5-Fr bipolar needle was performed in saline solution mimicking THL, while saline solution rinsing the ovaries after monopolar electrocoagulation.
The monopolar drilling caused more tissue damage than the bipolar needle in saline (p < 0.01). The ratio of the damage of monopolar electrocoagulation (40 w, 3s) over that of bipolar diathermy in saline solution (70 w, 15 s) was 7.4 [(16.74 +/- 1.30) mm(3)/(2.27 +/- 0.49) mm(3)]. In the bipolar groups, the 70 w power set (15s and 20 s) caused significantly more tissue damage than the 50 w ones (p < 0.05), and the amount of damage was not stimulation time dependent (p > 0.05).
The tissue damage caused by monopolar electrocoagulation with 40 w for 3 s was about seven times of that caused by 5-Fr bipolar one in saline with 70 w for 15 s . In THL drilling using a 5-Fr bipolar electrode, the current is more crucial than stimulation time.
评估在模拟经阴道注水腹腔镜（THL）的生理盐水环境中体外猪卵巢钻孔术对组织的损伤。
在新鲜猪卵巢上进行不同电流和激活时间的体外卵巢钻孔术，每组 15 个卵巢，每个卵巢 2 个穿刺点。使用模拟 THL 的生理盐水进行 5Fr 双极针钻孔，而在单极电凝后用生理盐水冲洗卵巢。
单极钻孔比生理盐水环境中的双极针造成更多的组织损伤（p < 0.01）。单极电凝（40w，3s）造成的损伤与双极电切（70w，15s）在生理盐水溶液中的比值为 7.4[（16.74 +/- 1.30）mm³/（2.27 +/- 0.49）mm³]。在双极组中，70w 功率设置（15s 和 20s）比 50w 功率设置（p < 0.05）造成的组织损伤明显更多，并且损伤量与刺激时间无关（p > 0.05）。
在模拟 THL 中使用 5Fr 双极电极进行钻孔时，40w 持续 3s 的单极电凝造成的组织损伤约为 70w 持续 15s 的 5Fr 双极针的 7 倍。电流比刺激时间对组织损伤更为关键。
