Marine Microbiology Laboratory of the Experimental Zooprophylactic Institute of Piemonte Liguria e Valle d'Aosta-La Spezia, Italy.
Lett Appl Microbiol. 2010 May;50(5):462-7. doi: 10.1111/j.1472-765X.2010.02820.x. Epub 2010 Feb 8.
The purpose of this work was to verify whether E. coli is a good indicator of viral contamination in mussels and Adenovirus could represent a better alternative as indicator organism of viral presence to guarantee consumer health protection.
Eighty samples of mussels from La Spezia Gulf were analysed for E. coli, Salmonella, Adenovirus, Norovirus and hepatitis A virus with cultural and biomolecular tests. The results of bacterial parameters showed E. coli within the law's limits and the absence of Salmonella. Twelve samples were positive for Adenovirus presence, one for Norovirus genogroup II and two for hepatitis A virus. None of these positive mussels was found to be contaminated with more than one virus at the same time.
This study showed that there was not a direct correlation between the presence of human pathogenic viruses and bacterial indicators.
Both E. coli and Adenovirus cannot be considered valid substitutes for the direct research of human pathogenic viruses in mussels. To improve consumer health protection, the European Commission will provide standardized methods for Norovirus and hepatitis A virus detection as soon as possible.
本研究旨在验证大肠杆菌是否可作为贝类中病毒污染的良好指示生物，而腺病毒作为指示生物来保证消费者健康是否更有优势。
采用传统培养法和分子生物学方法，对来自拉斯佩齐亚海湾的 80 份贻贝样本进行了大肠杆菌、沙门氏菌、腺病毒、诺如病毒和甲型肝炎病毒的检测。细菌参数结果显示，大肠杆菌在法律规定的范围内，且未检出沙门氏菌。12 份样本检测出腺病毒阳性，1 份样本检测出诺如病毒基因 II 型，2 份样本检测出甲型肝炎病毒。这些阳性贻贝样本中没有一种同时被检测出多种病毒。
本研究表明，人类致病性病毒的存在与细菌指标之间没有直接的相关性。
大肠杆菌和腺病毒均不能作为直接检测贝类中人类致病性病毒的有效替代物。为了提高消费者的健康保护水平，欧盟委员会将尽快提供诺如病毒和甲型肝炎病毒检测的标准化方法。
