Institute of Surgical Pathology, Universitäts Spital Zürich, Zurich, Switzerland.
BMC Urol. 2010 Feb 25;10:4. doi: 10.1186/1471-2490-10-4.
GOLPH2 (Golgi phosphoprotein 2) is a novel Golgi membrane protein. Despite its unknown physiologic function, however, it has been proposed as a biomarker for hepatocellular and prostate carcinoma due to its upregulation in those cancer entities. Whether the overexpression of GOLPH2 is tumour specific or a generic parameter of malignancy and whether this finding is true for additional carcinomas has not been determined. In this study, we aimed to evaluate the expression pattern of GOLPH2 in testicular seminomas, the most common histologic subtype of testicular neoplasm.
GOLPH2 protein expression was assessed by immunohistochemistry in 69 testicular seminomas and compared to the expression rates in matching normal testicular tissue and intratubular germ cell neoplasia of unclassified type (IGCNU). In addition, a subset of Leydig cell tumours was analyzed accordingly.
GOLPH2 was consistently overexpressed (89.9%) in seminomas. Matching non-neoplastic tissue showed weak or negative staining. The observed differences between non-neoplastic and neoplastic tissue were statistically highly significant (p < 0.001). There were no significant associations with tumour status. Interestingly, GOLPH2 was also highly expressed in the intertubular Leydig cells as well as in Leydig cell tumours.
GOLPH2 protein is highly expressed in seminomas and in Leydig cell tumours. This study fosters the association of GOLPH2 with malignant neoplastic processes. The staining pattern is easily assessable and consistent which is a favourable property especially in clinical settings. GOLPH2 could be a novel immunohistochemical marker for the assessment of testicular neoplasms, especially against the background that in analogy to hepatocellular carcinomas complementary GOLPH2 serum levels might be helpful in detecting metastases or recurrent tumour. Therefore serum studies and analyses of GOLPH2 expression in non-seminomatous germ cell tumours are strongly warranted.
GOLPH2（高尔基磷酸蛋白 2）是一种新型的高尔基膜蛋白。尽管其生理功能未知，但由于其在肝癌和前列腺癌中的上调，它被提议作为这些癌症的生物标志物。GOLPH2 的过表达是否是肿瘤特异性的，还是恶性肿瘤的一般参数，以及这一发现是否适用于其他癌症，目前尚未确定。在这项研究中，我们旨在评估 GOLPH2 在睾丸精原细胞瘤中的表达模式，睾丸精原细胞瘤是睾丸肿瘤中最常见的组织学亚型。
通过免疫组织化学法检测 69 例睾丸精原细胞瘤中的 GOLPH2 蛋白表达，并与匹配的正常睾丸组织和未分类型管内生殖细胞肿瘤（IGCNU）的表达率进行比较。此外，还相应地分析了一部分间质细胞瘤。
GOLPH2 在精原细胞瘤中一致过表达（89.9%）。匹配的非肿瘤组织显示弱阳性或阴性染色。非肿瘤组织和肿瘤组织之间的差异具有统计学意义（p < 0.001）。肿瘤状态之间没有显著的相关性。有趣的是，GOLPH2 在间质细胞瘤和间质细胞瘤中也高度表达。
GOLPH2 蛋白在精原细胞瘤和间质细胞瘤中高度表达。本研究促进了 GOLPH2 与恶性肿瘤过程的关联。该染色模式易于评估且一致，这是一个特别有利的特性，尤其是在临床环境中。GOLPH2 可能是一种新的用于评估睾丸肿瘤的免疫组织化学标志物，特别是在类似于肝细胞癌的情况下，互补的 GOLPH2 血清水平可能有助于检测转移或复发性肿瘤。因此，强烈需要进行血清研究，并分析非精原细胞瘤生殖细胞肿瘤中 GOLPH2 的表达。
