Department & Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul, Korea.
Cell Transplant. 2009;18(12):1359-68. doi: 10.3727/096368909X475329.
This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free drop contusion at the T9 level. The SCI rats were then classified into three groups; MSCs, NMSCs, and phosphate-buffered saline (PBS)-treated groups. The cells or PBS were administrated 1 week after SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scores were measured at 1-week intervals for 9 weeks. Somatosensory evoked potentials (SSEPs) and motor evoked potentials (MEPs) were also recorded 8 weeks after transplantation. While transplantation of MSCs led to a clear tendency of motor recovery, NMSC-treated rats had significantly improved BBB scores and showed significantly shortened initial latency, N1 latency, and P1 latency of the SSEPs compared to PBS controls. In addition, 5-bromo-2-deoxyuridine (BrdU)-prelabeled MSCs costained for BrdU and glial fibrillary acidic protein (GFAP) or myelin basic protein (MBP) were found rostrally and caudally 5 mm each from the epicenter of the necrotic cavity 4 weeks after transplantation. These results suggest that neurally differentiated cells might be an effective therapeutic source for functional recovery after SCI.
本研究旨在探讨骨髓间充质干细胞（MSCs）或神经分化的骨髓间充质干细胞（NMSCs）移植后在脊髓损伤（SCI）大鼠模型中的功能恢复情况。采用 NYU 撞击器在 T9 水平造成自由落体挫伤，使 Sprague-Dawley 大鼠发生不完全性 SCI。然后将 SCI 大鼠分为 MSCs、NMSCs 和磷酸盐缓冲盐水（PBS）处理组。细胞或 PBS 在 SCI 后 1 周给予。在 9 周的时间内，每隔 1 周测量 1 次巴索-比蒂-布雷森汉（BBB）运动评分。移植后 8 周还记录体感诱发电位（SSEP）和运动诱发电位（MEP）。虽然 MSC 移植导致运动恢复有明显趋势，但 NMSC 治疗的大鼠 BBB 评分明显提高，SSEP 的初始潜伏期、N1 潜伏期和 P1 潜伏期明显缩短，与 PBS 对照组相比。此外，在移植后 4 周，距坏死腔中心 5mm 处的头端和尾端均发现 BrdU 预标记的 MSC 与 BrdU 和神经胶质纤维酸性蛋白（GFAP）或髓鞘碱性蛋白（MBP）共染色。这些结果表明，神经分化细胞可能是 SCI 后功能恢复的有效治疗来源。
