Cho Sung-Rae, Kim Yong Rae, Kang Hoi-Sung, Yim Sun Hee, Park Chang-Il, Min Yoo Hong, Lee Bae Hwan, Shin Ji Cheol, Lim Jong-Baeck
Department & Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, Seoul, Korea.
Department of Rehabilitation Medicine, Pochun Joongmoon University College of Medicine, Seoul, Korea.
Cell Transplant. 2016 Jul;25(7):1423. doi: 10.3727/096368916X692078.
This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free drop contusion at the T9 level. The SCI rats were then classified into three groups; MSCs, NMSCs, and phosphate-buffered saline (PBS)-treated groups. The cells or PBS were administrated 1 week after SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scores were measured at 1-week intervals for 9 weeks. Somatosensory evoked potentials (SSEPs) and motor evoked potentials (MEPs) were also recorded 8 weeks after transplantation. While transplantation of MSCs led to a clear tendency of motor recovery, NMSC-treated rats had significantly improved BBB scores and showed significantly shortened initial latency, N1 latency, and P1 latency of the SSEPs compared to PBS controls. In addition, 5-bromo-2-deoxyuridine (BrdU)-prelabeled MSCs costained for BrdU and glial fibrillary acidic protein (GFAP) or myelin basic protein (MBP) were found rostrally and caudally 5 mm each from the epicenter of the necrotic cavity 4 weeks after transplantation. These results suggest that neurally differentiated cells might be an effective therapeutic source for functional recovery after SCI.
本研究旨在探讨在大鼠脊髓损伤（SCI）模型中，移植骨髓来源的间充质干细胞（MSC）或神经分化的间充质干细胞（NMSC）后的功能恢复情况。使用纽约大学撞击器对Sprague-Dawley大鼠造成T9水平的自由落体挫伤，导致不完全性SCI。然后将SCI大鼠分为三组：MSC组、NMSC组和磷酸盐缓冲盐水（PBS）处理组。在SCI后1周给予细胞或PBS。在9周内每隔1周测量Basso-Beattie-Bresnahan（BBB）运动评分。移植后8周还记录体感诱发电位（SSEP）和运动诱发电位（MEP）。虽然MSC移植导致运动恢复有明显趋势，但与PBS对照组相比，NMSC处理的大鼠BBB评分显著改善，SSEP的初始潜伏期、N1潜伏期和P1潜伏期显著缩短。此外，移植后4周，在坏死腔中心头侧和尾侧各5 mm处发现5-溴-2-脱氧尿苷（BrdU）预标记的MSC与BrdU和胶质纤维酸性蛋白（GFAP）或髓鞘碱性蛋白（MBP）共染色。这些结果表明，神经分化细胞可能是SCI后功能恢复的有效治疗来源。
