Department of Biochemistry and Molecular Biology, University of Parma, 43124 Parma, Italy.
Anal Biochem. 2010 Jun 15;401(2):266-70. doi: 10.1016/j.ab.2010.02.025. Epub 2010 Feb 23.
Dynamic light scattering (DLS) is a technique capable of determining the hydrodynamic radius of proteins. From this parameter, a molecular weight can be assessed provided that an appropriate calibration curve is available. To this goal, a globin-based calibration curve was used to determine the polymerization state of a recombinant hemoglobin-based oxygen carrier and to assess the equivalent molecular weight of hemoglobins conjugated with polyethylene glycol molecules. The good agreement between DLS values and those obtained from gel filtration chromatography is a consequence of the high similarity in structure, shape, and density within the globin superfamily. Moreover, globins and heme proteins in general share similar spectroscopic properties, thereby reducing possible systematic errors associated with the absorption of the probe radiation by the chromophore.
动态光散射（DLS）是一种能够确定蛋白质流体力学半径的技术。通过该参数，可以评估分子量，前提是有合适的校准曲线。为此，使用基于球蛋白的校准曲线来确定重组血红蛋白基氧载体的聚合状态，并评估与聚乙二醇分子缀合的血红蛋白的等效分子量。DLS 值与凝胶过滤色谱法获得的值之间的良好一致性是由于球蛋白超家族在结构、形状和密度方面具有高度相似性。此外，球蛋白和血红素蛋白通常具有相似的光谱特性，从而减少了与发色团吸收探针辐射相关的可能系统误差。
