Alomari Esra'a, Bruno Stefano, Ronda Luca, Paredi Gianluca, Bettati Stefano, Mozzarelli Andrea
Department of Food and Drug, University of Parma, Parma, Italy.
Biopharmanet-TEC, University of Parma, Parma, Italy.
Data Brief. 2018 Jul 3;19:2215-2220. doi: 10.1016/j.dib.2018.06.088. eCollection 2018 Aug.
The data reported here are a comparison among four different methods for the detection of carbonylated proteins, a validated biomarker of oxidative stress. The reference samples were heart and kidney extracts of Guinea pigs transfused with hemoglobin-based oxygen carriers (Alomari et al. FRBM, [11]). We measured the carbonyl content of organ extracts by using i) the Levine spectrophotometric method, which takes advantage of the chromogenic reaction of carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH), ii) a commercially available ELISA assay based on an anti-DNPH antibodies, iii) a commercially available Western blot method based on anti-DNPH antibodies and iv) an in-gel detection approach with the fluorophoric reagent fluorescein-5-thiosemicarbazide. The former two methods measure total protein carbonylation of a sample, whereas the latter two require an electrophoretic separation and therefore potentially allow for the identification of specific carbonylated proteins.
本文报道的数据是对四种不同的羰基化蛋白质检测方法的比较，羰基化蛋白质是一种经过验证的氧化应激生物标志物。参考样品是输注基于血红蛋白的氧载体的豚鼠的心脏和肾脏提取物（Alomari等人，《自由基生物学与医学》，[11]）。我们使用以下方法测量器官提取物中的羰基含量：i）莱文分光光度法，该方法利用羰基与2,4-二硝基苯肼（DNPH）的显色反应；ii）基于抗DNPH抗体的市售ELISA检测法；iii）基于抗DNPH抗体的市售蛋白质印迹法；iv）使用荧光试剂5-硫代半卡巴腙荧光素的凝胶内检测方法。前两种方法测量样品中的总蛋白质羰基化，而后两种方法需要进行电泳分离，因此有可能识别特定的羰基化蛋白质。
