Department of Forensic Medicine and Tampere University Hospital, FI-33014 University of Tampere, Finland.
J Mol Biol. 2010 Apr 16;397(5):1144-55. doi: 10.1016/j.jmb.2010.02.029. Epub 2010 Feb 23.
We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.
我们利用电镜和针对 RNA/DNA 杂交的抗体免疫纯化的方法证实，完整的线粒体 DNA 复制中间体在其全长上基本上都是双链的，但在一条链上含有广泛的 RNA 片段。然而，在这些分子中 RNA 的保存程度高度依赖于所使用的制备方法。这些发现强烈支持涉及整个滞后链 RNA 掺入的线粒体 DNA 复制的链偶联模型。
