纤维蛋白片段在肝癌细胞合成纤维蛋白原过程中的假定作用。

The putative role of fibrin fragments in the biosynthesis of fibrinogen by hepatoma cells.

作者信息

Wang Y, Fuller G M

机构信息

Department of Cell Biology, University of Alabama, Birmingham 35294.

出版信息

Biochem Biophys Res Commun. 1991 Mar 15;175(2):562-7. doi: 10.1016/0006-291x(91)91602-9.

DOI:10.1016/0006-291x(91)91602-9

PMID:2018504

Abstract

The current study reinvestigated whether pure plasminolytic fragments D and E from fibrin directly increase fibrinogen synthesized by the hepatocyte. Fibrinogen protein levels and fibrinogen mRNA levels in an interleukin-6 (IL-6) responsive rat hepatoma cell line (FAZA) were measured quantitatively by [35S]-methionine pulse-chase experiments and Northern hybridizations, respectively. The results demonstrate that neither fragments D nor E, alone or in combination, in the presence or absence of dexamethasone, had any influence on the production of fibrinogen.

摘要

本研究再次探讨了来自纤维蛋白的纯纤溶片段D和E是否能直接增加肝细胞合成的纤维蛋白原。分别通过[35S] - 蛋氨酸脉冲追踪实验和Northern杂交定量检测了白细胞介素 - 6（IL - 6）反应性大鼠肝癌细胞系（FAZA）中的纤维蛋白原蛋白水平和纤维蛋白原mRNA水平。结果表明，无论是片段D还是片段E，单独或联合使用，无论是否存在地塞米松，对纤维蛋白原的产生均无任何影响。