Ritchie D G, Levy B A, Adams M A, Fuller G M
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1530-4. doi: 10.1073/pnas.79.5.1530.
The effect of plasmin-derived fibrinogen fragments on the biosynthesis of fibrinogen was investigated in cultured monolayers of rat hepatocytes. Incubating the cells with several concentrations of either fibrinogen or fibrin fragment D or E had no effect on the synthesis and secretion of fibrinogen by these cells. However, if the fragments were incubated with isolated peripheral blood leukocytes, they caused these cells to secrete a factor that when added to the hepatocytes caused an increase in fibrinogen synthesis 4- to 6-fold over controls. Moreover, the hepatocyte-stimulating factor also affected the production of several other proteins produced by the hepatocyte. These results demonstrate that both fragments D and E can stimulate hepatic fibrinogen synthesis via an indirect leukocyte-mediated pathway.
在大鼠肝细胞单层培养物中研究了纤溶酶衍生的纤维蛋白原片段对纤维蛋白原生物合成的影响。用几种浓度的纤维蛋白原或纤维蛋白片段D或E孵育细胞，对这些细胞纤维蛋白原的合成和分泌没有影响。然而，如果将这些片段与分离的外周血白细胞一起孵育，它们会使这些细胞分泌一种因子，当将该因子添加到肝细胞中时，会导致纤维蛋白原合成比对照增加4至6倍。此外，肝细胞刺激因子也影响肝细胞产生的其他几种蛋白质的产生。这些结果表明，片段D和E都可以通过间接的白细胞介导途径刺激肝脏纤维蛋白原的合成。
