Department of Biology, Dowling College, 150 Idle Hour Blvd., Oakdale, NY 11769, USA.
J Hazard Mater. 2010 Jun 15;178(1-3):1141-5. doi: 10.1016/j.jhazmat.2010.01.141. Epub 2010 Feb 4.
White rot fungi are one of the key group of microorganisms that help to enrich the soil via degradation of wood. In the current communication, influence of iron and copper nanoparticles on the production of lignocellulolytic enzymes by Trametes versicolor have been investigated. The production of enzymes in the presence of the two nanoparticles was compared to that of ferrous and cupric ions respectively. Results show that both the tested nanoparticles alter the production profile of the lignocellulolytic enzymes when compared to the control set. The production of laccase was not influenced by iron nanoparticles but was effected by copper nanoparticles within 24h of incubation. Both the nanoparticles decreased the production of beta-glucosidase, beta-xylosidase and cellobiohydrolase significantly. However, the production profile of Mn-peroxidase and remained statistically similar to that of control when the organism was incubated with iron and copper nanoparticles. The production profiles were also different when one compares the ionic form of metals and the nanoparticles, suggesting different mechanism of action of the particles on the organism. The difference in the production profile was not growth related as no significant difference was recorded for either form of iron and copper on the growth of T. versicolor.
白腐真菌是有助于通过降解木材使土壤富化的关键微生物类群之一。在本研究中，研究了铁和铜纳米粒子对糙皮侧耳产木质纤维素酶的影响。将两种纳米粒子存在下的酶生产与亚铁和铜离子的生产进行了比较。结果表明，与对照组相比，两种纳米粒子均改变了木质纤维素酶的生产曲线。铁纳米粒子对漆酶的产生没有影响，但铜纳米粒子在孵育 24 小时内对其产生了影响。两种纳米粒子均显著降低了β-葡萄糖苷酶、β-木糖苷酶和纤维二糖水解酶的产生。然而，当用铁和铜纳米粒子孵育时，Mn-过氧化物酶的产生曲线与对照相比在统计学上仍相似。当比较金属的离子形式和纳米粒子时，产酶曲线也不同，这表明粒子对生物体的作用机制不同。由于在 T. versicolor 的生长中没有记录到铁和铜的任何一种形式有显著差异，因此产酶曲线的差异与生长无关。
