Technische Universität Berlin, Institute of Biotechnology, Department of Bioprocess Engineering, Ackerstr. 71-76, D-13355 Berlin, Germany.
Curr Opin Biotechnol. 2010 Feb;21(1):114-21. doi: 10.1016/j.copbio.2010.02.001. Epub 2010 Feb 23.
Analytical approaches for a comprehensive understanding of the metabolic networks in microbial cultures are mostly based on small-scale cultures which are in a steady state or undergo dynamic changes. For drawing conclusions to industrial-scale bioprocesses, however, it is important to understand that cells in large-scale bioreactors are exposed steadily to fast changes, because of an inhomogeneous environment. Analytical approaches that aim for large-scale bioprocess understanding need to apply specific laboratory simulators. Recent developments in cell cultivation techniques and computational tools provide improved possibilities to evaluate how a process will behave in the final scale. These simulators will pave the way for screening robust strains and process conditions.
分析方法旨在全面了解微生物培养物中的代谢网络，这些方法主要基于处于稳定状态或经历动态变化的小规模培养物。然而，要得出关于工业规模生物工艺的结论，必须了解到，由于环境不均匀，大规模生物反应器中的细胞会不断受到快速变化的影响。旨在了解大规模生物工艺的分析方法需要应用特定的实验室模拟器。细胞培养技术和计算工具的最新发展为评估工艺在最终规模下的表现提供了更好的可能性。这些模拟器将为筛选稳健的菌株和工艺条件铺平道路。
