Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123 Saarbrücken, Germany.
Curr Opin Biotechnol. 2010 Feb;21(1):63-9. doi: 10.1016/j.copbio.2010.01.011. Epub 2010 Feb 15.
Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants. While MFA is widely established and applied in microorganisms, it remains still a challenge to adapt these methods to eukaryotic cell systems having a higher complexity particularly concerning compartmentation or media composition. In fungi MFA was used in the past few years to analyze a variety of conditions and factors and their effects on cellular metabolism. In mammalian cells MFA was applied mainly in cell culture technology and in medical and toxicological research. (13)C metabolic studies on native whole plants are additionally challenging by the fact that CO(2) is usually the only carbon source.
代谢通量分析（MFA）是真核细胞系统生物学研究的有力工具。本文综述了代谢通量分析在真菌、哺乳动物细胞和植物中的最新进展、挑战和应用。虽然 MFA 在微生物中已被广泛建立和应用，但将这些方法应用于具有更高复杂性的真核细胞系统，特别是在涉及区室化或培养基组成方面，仍然具有挑战性。在过去的几年中，真菌中的 MFA 被用于分析各种条件和因素及其对细胞代谢的影响。在哺乳动物细胞中，MFA 主要应用于细胞培养技术以及医学和毒理学研究。由于 CO2 通常是唯一的碳源，因此对天然全植物进行 13C 代谢研究具有额外的挑战性。
