CNISM and Università di Napoli Federico II, Dipartimento di Scienze Fisiche, Complesso Universitario Monte S. Angelo, Via Cinthia, I-80126 Napoli, Italy.
Phys Med. 2010 Oct;26(4):233-9. doi: 10.1016/j.ejmp.2010.02.001. Epub 2010 Feb 24.
Raman confocal microscopy, combined with an optical stretcher, is used to study the spatial distribution and the oxidation state of hemoglobin in erythrocytes under stretching condition. In particular, a near infrared laser (λ = 1064 nm) is used to generate multiple time-sharing Optical Tweezers to trap and stretch a single erythrocyte, while a second laser (λ = 532 nm) acts as Raman probe. Our study demonstrates that stretching induces hemoglobin transition to the deoxygenated state. Moreover, by using Principal Component Analysis we prove the reversibility of the oxy ↦ deoxy hemoglobin transition after application of the optically induced mechanical stress.
拉曼共聚焦显微镜结合光镊，用于研究拉伸条件下红细胞中血红蛋白的空间分布和氧化状态。具体来说，使用近红外激光（λ=1064nm）产生多个分时光镊来捕获和拉伸单个红细胞，而第二个激光（λ=532nm）用作拉曼探针。我们的研究表明，拉伸会导致血红蛋白向去氧状态转变。此外，通过使用主成分分析，我们证明了在施加光致机械应力后，氧合血红蛋白向去氧血红蛋白的转变是可逆的。
