Department of Obstetrics and Gynecology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.
Reproduction. 2010 May;139(5):847-56. doi: 10.1530/REP-08-0366. Epub 2010 Feb 25.
It has been widely accepted that serine/threonine protein phosphatases (PPPs) are associated with the regulation of sperm hyperactivation. In the present study, we examined the types of PPPs associated with the regulation of hamster sperm hyperactivation. Protein phosphatases PPP1CA, PPP1CC, PPP2, and PPP3 are present in hamster sperm. In the experiments using several inhibitors, sperm hyperactivation was enhanced when PPP2 was inhibited at least, although inhibition of PPP1 also enhanced sperm hyperactivation. Interestingly, sperm were hyperactivated after PPP2 became an inactive form. And then, PPP1CA became an active form after sperm were hyperactivated. It has also been widely accepted that tyrosine phosphorylation is closely associated with the regulation of sperm hyperactivation. When PPP2 was inhibited, tyrosine phosphorylation was not enhanced at all. On the other hand, inhibition of PPP1 enhanced tyrosine phosphorylation. From the results, it is likely that PPP2 is closely associated with the regulation of sperm hyperactivation, although it is not associated with the regulation of tyrosine phosphorylation.
丝氨酸/苏氨酸蛋白磷酸酶（PPPs）与精子超激活的调节有关，这已得到广泛认可。在本研究中，我们研究了与调控仓鼠精子超激活相关的 PPP 类型。蛋白磷酸酶 PPP1CA、PPP1CC、PPP2 和 PPP3 存在于仓鼠精子中。在使用几种抑制剂的实验中，至少当抑制 PPP2 时，精子超激活增强，尽管抑制 PPP1 也增强了精子超激活。有趣的是，PPP2 变成无活性形式后，精子超激活。然后，PPP1CA 在精子超激活后变成活性形式。酪氨酸磷酸化与精子超激活的调节密切相关，这也得到了广泛认可。当抑制 PPP2 时，酪氨酸磷酸化根本没有增强。另一方面，抑制 PPP1 增强了酪氨酸磷酸化。从结果来看，尽管 PPP2 与酪氨酸磷酸化的调节无关，但它与精子超激活的调节密切相关。
