Department of Molecular Pathology, Nara Medical University School of Medicine, Kashihara, Nara, 634-8521, Japan.
Pathobiology. 2010;77(1):17-23. doi: 10.1159/000272950. Epub 2010 Feb 25.
Cancer-secreted high mobility group 1 (HMGB1) induces apoptosis of macrophages and suppresses the host anti-cancer immune system.
We here examined the effect of HMGB1 on macrophages in the lymph nodes of colorectal cancer (CRC) patients.
Regional lymph nodes of 50 Dukes C CRCs were compared with 50 Dukes B CRCs.
Dukes C tumors exhibited higher HMGB1 labeling indices and higher HMGB1 concentrations in primary tumors than Dukes B CRCs. Macrophages in the regional lymph nodes were decreased in non-metastasized nodes as well as metastasized nodes in Dukes C cases, whereas macrophage numbers in Dukes B nodes were higher than in Dukes C nodes. Nodal HMGB1 concentration was higher in Dukes C nodes than in Dukes B nodes, being inversely correlated with macrophage numbers. Nodal HMGB1 concentration was correlated with HMGB1 concentration and lymph vessel density in the primary tumors.
These data suggest that HMGB1 secreted from primary tumors spread to the regional lymph nodes decreases the number of macrophages to attenuate the anti-metastatic defense of the lymph nodes in patients with CRCs.
癌症分泌的高迁移率族蛋白 1（HMGB1）诱导巨噬细胞凋亡，并抑制宿主的抗肿瘤免疫。
本研究旨在探讨 HMGB1 对结直肠癌（CRC）患者淋巴结中巨噬细胞的影响。
对 50 例 Dukes C CRC 患者的区域淋巴结与 50 例 Dukes B CRC 患者的区域淋巴结进行比较。
与 Dukes B CRC 相比，Dukes C 肿瘤的 HMGB1 标记指数和原发肿瘤中的 HMGB1 浓度更高。在非转移性和转移性 Dukes C 病例的区域淋巴结中，巨噬细胞数量减少，而 Dukes B 病例的巨噬细胞数量高于 Dukes C 病例。淋巴结中 HMGB1 的浓度在 Dukes C 淋巴结中高于 Dukes B 淋巴结，与巨噬细胞数量呈负相关。淋巴结中 HMGB1 的浓度与原发肿瘤中的 HMGB1 浓度和淋巴管密度相关。
这些数据表明，源自原发性肿瘤的 HMGB1 扩散到区域淋巴结，减少了巨噬细胞的数量，从而削弱了 CRC 患者淋巴结的抗肿瘤转移防御能力。
