Department of Biology & Medicinal Science, Pai Chai University, Daejeon, 302-735, Republic of Korea.
Plant Foods Hum Nutr. 2010 Jun;65(2):91-8. doi: 10.1007/s11130-009-0146-8.
The phenolic compounds and flavonoids were determined from the extracts of Withania somnifera root (WSREt) and leaf (WSLEt). The WSREt has 28.26 mg/g total phenolic compounds and 17.32 mg/g flavonoids, whereas WSLEt has 5.4 mg/g total phenolic compounds and 5.1 mg/g flavonoids. The WSREt, WSLEt and glibenclamide were orally administered daily to diabetic rats for 8 weeks. After the treatment, the levels of urine sugar, blood glucose, liver glycogen, and antioxidants like vitamin C and E in plasma and superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) in liver, kidney and heart were determined. Diabetic rats showed a significant (p < 0.05) elevation in glucose and TBARS and a significant (p < 0.05) reduction in glycogen, vitamin C and E, SOD, CAT, GPx, GST, and GSH levels when compared to normal control rats. Administration of WSREt, WSLEt and glibenclamide to diabetic rats restored the levels to normal. In the light of aforesaid facts, it is suggested that the presence of phenolic compounds including flavonoids in W. somnifera root and leaf extracts and their antioxidant activity may play a vital role in reduction of blood glucose level in alloxan-induced diabetic rats.
从茄科植物根（WSREt）和叶（WSLEt）的提取物中测定了酚类化合物和类黄酮。WSREt 含有 28.26mg/g 的总酚类化合物和 17.32mg/g 的类黄酮，而 WSLEt 含有 5.4mg/g 的总酚类化合物和 5.1mg/g 的类黄酮。WSREt、WSLEt 和格列本脲每天口服给予糖尿病大鼠 8 周。治疗后，测定尿液糖、血糖、肝糖原以及血浆中维生素 C 和 E 等抗氧化剂和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、硫代巴比妥酸反应物质（TBARS）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）和还原型谷胱甘肽（GSH）在肝、肾和心脏中的水平。与正常对照组大鼠相比，糖尿病大鼠的血糖和 TBARS 显著升高（p<0.05），肝糖原、维生素 C 和 E、SOD、CAT、GPx、GST 和 GSH 水平显著降低（p<0.05）。给予 WSREt、WSLEt 和格列本脲可使糖尿病大鼠的这些水平恢复正常。根据上述事实，建议茄科植物根和叶提取物中存在酚类化合物（包括类黄酮）及其抗氧化活性可能在降低丙烯醛诱导的糖尿病大鼠血糖水平方面发挥重要作用。
