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基于白蛋白的纳米载体释放系统对多孔聚乙烯植入物的表面改性

Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System.

作者信息

Eckrich Jonas, Hoormann Niklas, Kersten Erik, Piradashvili Keti, Wurm Frederik R, Heller Martin, Becker Sven, Anusic Toni, Brieger Juergen, Strieth Sebastian

机构信息

Department of Otorhinolaryngology, University Medical Center Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany.

Department of Otorhinolaryngology, University Medical Center Bonn (UKB), Venusberg-Campus 1, 53127 Bonn, Germany.

出版信息

Biomedicines. 2021 Oct 16;9(10):1485. doi: 10.3390/biomedicines9101485.

DOI:10.3390/biomedicines9101485
PMID:34680602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8533240/
Abstract

BACKGROUND

Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo.

METHODS

Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation.

RESULTS

Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application.

CONCLUSIONS

Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.

摘要

背景

多孔聚乙烯（PPE）植入物用于组织缺损的重建，但如果向宿主组织内生长不足则有排斥风险。多种生长因子可促进植入物向内生长，然而长期梯度是介导这些效应的先决条件。由于用纳米载体对植入物表面进行修饰可通过持续释放因子促进长期梯度形成，因此对用交联白蛋白纳米载体修饰的植入物进行了体内评估。

方法

通过反相微乳液技术制备来自小鼠血清白蛋白（MSA）的纳米载体，其包裹低摩尔质量或高摩尔质量的荧光物质。随后用这些纳米载体包被PPE植入物。在对照队列中，通过浸涂用同源的未包裹的物质包被植入物。植入后，利用小鼠背部皮褶腔，通过重复荧光显微镜对植入物进行为期10天的体内分析。

结果

用MSA纳米载体修饰植入物可显著延长包裹的小分子的存在时间，而在研究时间范围内，无论应用形式如何，均可检测到大分子。

结论

用MSA纳米载体对PPE植入物进行表面修饰可改变释放动力学，尤其是在使用小分子物质时，因此可实现延长因子释放以促进植入物整合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/2b390872a896/biomedicines-09-01485-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/9b28d76e0dbc/biomedicines-09-01485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/05f35aab5347/biomedicines-09-01485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/1c416f11cc67/biomedicines-09-01485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/731d25713639/biomedicines-09-01485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/161ad4ac8f10/biomedicines-09-01485-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/0be72debb56f/biomedicines-09-01485-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/7a0e0b8bb69d/biomedicines-09-01485-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/2b390872a896/biomedicines-09-01485-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/9b28d76e0dbc/biomedicines-09-01485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/05f35aab5347/biomedicines-09-01485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/1c416f11cc67/biomedicines-09-01485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/731d25713639/biomedicines-09-01485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/161ad4ac8f10/biomedicines-09-01485-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/0be72debb56f/biomedicines-09-01485-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/7a0e0b8bb69d/biomedicines-09-01485-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/117d/8533240/2b390872a896/biomedicines-09-01485-g008.jpg

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