Pharmaceutics and Biopharmaceutics Division, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany.
J Biomed Mater Res A. 2010 Jul;94(1):298-307. doi: 10.1002/jbm.a.32703.
For successful bone tissue engineering, scaffolds with tailored properties are a basic requirement. The combination of different available materials not only appears to be desirable but also very challenging. In this study, a composite material consisting of hydroxyapatite and collagen was produced by a biomimetic precipitation method and characterized by X-ray diffraction (XRD) and thermogravimetry (TGA). Subsequently, a suspension-quick-freezing and lyophilization method was used to incorporate the hydroxyapatite into a polymeric matrix consisting of collagen and chitosan. Before physicochemical characterization, the highly porous scaffolds were consolidated by a dehydrothermal treatment (DHT). The main attention was focused on the particle size of hydroxyapatite, which should be in the nanometer range. This is relevant to achieve a homogeneous resorption of the material by osteoclasts. Small-angle X-ray scattering (SAXS), atomic force microscopy (AFM), and environmental scanning electron microscopy (ESEM) were used to evaluate the outcome. The results suggest a successful polymeric embedding of nanoscaled hydroxyapatite particles into the matrix of the spongy construct. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
为了成功进行骨组织工程，具有定制特性的支架是基本要求。结合不同可用材料不仅看起来是可取的，而且极具挑战性。在这项研究中，通过仿生沉淀法制备了一种由羟基磷灰石和胶原蛋白组成的复合材料，并通过 X 射线衍射（XRD）和热重分析（TGA）进行了表征。随后，采用悬浮快速冷冻和冻干法将羟基磷灰石掺入由胶原蛋白和壳聚糖组成的聚合物基质中。在进行物理化学表征之前，通过脱水热处理（DHT）对高多孔支架进行了固结。主要关注的是羟基磷灰石的粒径，应为纳米级。这对于通过破骨细胞实现材料的均匀吸收很重要。小角 X 射线散射（SAXS）、原子力显微镜（AFM）和环境扫描电子显微镜（ESEM）用于评估结果。结果表明，纳米级羟基磷灰石颗粒成功地嵌入了海绵状结构的基质中。（c）2010 年 Wiley 期刊，Inc. J Biomed Mater Res，2010。
