Wang Yan, Zhang Lihai, Hu Min, Liu Hongchen, Wen Weisheng, Xiao Hongxi, Niu Yu
Department of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, People's Republic of China.
J Biomed Mater Res A. 2008 Jul;86(1):244-52. doi: 10.1002/jbm.a.31758.
In this study, a new artificial bone matrix was constructed with collagen and Ca(5)(PO(4))(3)OH (hydroxyapatite/HA) which are the main components of natural bone. To improve the property of the artificial bone matrix, chitosan (CS), a kind of positive charged polysaccharide, was crosslinked into the scaffolds. Solid-liquid phase separation method was used to shape 3D porous structure benefited for cells growing into. The artificial bone matrix was investigated by transmission electron microscopy, scanning electron microscopy, and electron spectroscopy for chemical analysis, etc. for structures and characteristics. And its ability of bone repair was investigated by orthotope bone defect reparation in vivo. The results showed that the artificial bone matrix was a porousscaffold with three-dimension interconnected fiber microstructure. HA particles were dispersed evenly among collagen fiber and CS was modified on the surface of collagen fiber. It was indicated that this artificial bone matrix could be used as a bone substitute with outstanding properties.
在本研究中，采用天然骨的主要成分胶原蛋白和Ca(5)(PO(4))(3)OH（羟基磷灰石/HA）构建了一种新型人工骨基质。为改善人工骨基质的性能，将一种带正电荷的多糖壳聚糖（CS）交联到支架中。采用固液相分离法塑造有利于细胞长入的三维多孔结构。通过透射电子显微镜、扫描电子显微镜和化学分析电子能谱等手段对人工骨基质的结构和特性进行了研究。并通过体内原位骨缺损修复研究了其骨修复能力。结果表明，人工骨基质是一种具有三维相互连接纤维微观结构的多孔支架。HA颗粒均匀分散在胶原纤维之间，CS修饰在胶原纤维表面。表明这种人工骨基质可作为一种性能优异的骨替代物。
