Department of Biomedical Engineering, McGill University, Duff Medical Building, 3775 University Street, Montreal, Quebec H3A 2B4, Canada.
J Biomed Mater Res A. 2010 Aug;94(2):419-25. doi: 10.1002/jbm.a.32688.
The main concern associated with metal-on-metal (MM) hip prosthesis is the presence of metal ions, mainly chromium (Cr) and cobalt (Co), which are found both systemically and locally in the organism of patients. Previous studies revealed that Cr(III) and Co(II) ions could induce damages to proteins in macrophage-like cells in vitro, probably through the formation of reactive oxygen species (ROS). We then hypothesized that these ions can modify the expression of antioxidant enzymes in these cells. Results showed that Cr(VI) induced the protein expression of Mn-superoxide dismutase, Cu/Zn-superoxide dismutase, catalase, glutathione peroxidase, and heme oxygenase-1 (HO-1) but had no effect of the expression of their mRNA. Cr(III) have no effect on the expression of all these antioxidant enzymes. Co(II) induced the expression of both protein and mRNA of HO-1 only. In conclusion, results showed that Cr(VI), Cr(III), and Co(II) had differential effects on the expression of antioxidant enzymes in macrophage-like cells in vitro.
与金属对金属（MM）髋关节假体相关的主要问题是金属离子的存在，主要是铬（Cr）和钴（Co），这些金属离子在患者的机体中既存在于全身也存在于局部。先前的研究表明，Cr(III)和 Co(II)离子可能会在体外诱导巨噬细胞样细胞中的蛋白质损伤，这可能是通过形成活性氧（ROS）。因此，我们假设这些离子可以修饰这些细胞中抗氧化酶的表达。结果表明，Cr(VI)诱导 Mn-超氧化物歧化酶、Cu/Zn-超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和血红素加氧酶-1（HO-1）的蛋白表达，但对其 mRNA 的表达没有影响。Cr(III)对所有这些抗氧化酶的表达均无影响。Co(II)仅诱导 HO-1 的蛋白和 mRNA 的表达。总之，结果表明，Cr(VI)、Cr(III)和 Co(II)对体外巨噬细胞样细胞中抗氧化酶的表达有不同的影响。
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