Department of Bromatology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, Zwirki i Wigury 101, 02-089 Warsaw, Poland.
Nutrients. 2022 Mar 18;14(6):1285. doi: 10.3390/nu14061285.
A study was conducted to determine the effect of long-term supplementation with selenium and copper, administered at twice the level used in the standard diet of rats, on the content of selected elements in the femoral bones of healthy rats and rats with implanted LNCaP cancer cells. After an adaptation period, the animals were randomly divided into two experimental groups. The rats in the experimental group were implanted with prostate cancer cells. The rats in the control group were kept in the same conditions as those in the experimental group and fed the same diet, but without implanted cancer cells. The cancer cells (LNCaP) were intraperitoneally implanted in the amount of 1 × 10 (in PBS 0.4 mL) at the age of 90 days. The content of elements in the samples was determined by a quadrupole mass spectrometer with inductively coupled plasma ionization (ICP-MS). In the femoral bones of rats with implanted LNCaP cells, in the case of the standard diet and the copper-enriched diet, there was a marked decreasing trend in the content of the analysed elements relative to the control rats. This may indicate slow osteolysis taking place in the bone tissue. Contrasting results were obtained for the diet enriched with selenium; there was no significant reduction in the level of these elements, and there was even an increase in the concentrations of Fe and K in the bones of rats with implanted LNCaP cells. Particularly, numerous changes in the mineral composition of the bones were generated by enriching the diet with copper. The elements that most often underwent changes (losses) in the bones were cobalt, iron, manganese and molybdenum. The changes observed, most likely induced by the implantation of LNCaP cells, may indicate a disturbance of mineral homeostasis.
一项研究旨在确定长期补充硒和铜（剂量为大鼠标准饮食中使用量的两倍）对健康大鼠和植入 LNCaP 癌细胞大鼠股骨中选定元素含量的影响。经过适应期后，动物被随机分为两组实验组。实验组大鼠被植入前列腺癌细胞。对照组大鼠保持与实验组相同的条件并喂食相同的饮食，但不植入癌细胞。癌细胞（LNCaP）以 1×10（在 0.4 mL PBS 中）的量腹腔内植入，年龄为 90 天。使用电感耦合等离子体质谱法（ICP-MS）的四极杆质谱仪测定样品中的元素含量。在植入 LNCaP 细胞的大鼠股骨中，在标准饮食和富铜饮食的情况下，与对照组大鼠相比，分析元素的含量呈明显下降趋势。这可能表明骨组织中发生了缓慢的溶骨作用。富含硒的饮食则得出了相反的结果；这些元素的水平没有显著降低，甚至在植入 LNCaP 细胞的大鼠的骨骼中 Fe 和 K 的浓度增加。特别地，饮食中富铜会导致骨骼中矿物质组成发生大量变化。在骨骼中最常发生变化（损失）的元素是钴、铁、锰和钼。这些观察到的变化，很可能是由 LNCaP 细胞的植入引起的，可能表明矿物质稳态的紊乱。
