College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2013 Apr;157(3):310-21. doi: 10.1016/j.cbpc.2013.02.003. Epub 2013 Feb 24.
Hexavalent chromium (Cr(6+)) is a common pollutant transient metal with high toxicity in the environment. The toxicological effects partly result from oxidative damage due to the production of excessive reactive oxygen species (ROS) in the reductive process of Cr(6+). To explore the influence of ROS induced directly by Cr(6+) on the oxidative stress generation and antioxidant system, the full length cDNAs of antioxidant-related genes cat, gpx1 and Cu/Zn-sod were successfully acquired from pengze crucian carp first and analyzed. Furthermore, the mRNA expression of the antioxidant genes encompassing catalase (cat), copper/zinc superoxide dismutase (Cu/Zn-sod) and glutathione peroxidase (gpx1), antioxidant enzyme activities of CAT, SOD, and GPx and total protein content were further studied in the gill, intestine and liver of pengze crucian carp (Carassius auratus var. Pengze) juveniles upon acute exposure to Cr(6+) at concentrations of 0.1, 1.0, 10 and 100 mg/L for 4 days. Differential significant changes of the antioxidant enzymes and gene expression were observed in different tissues. The findings contribute to better understanding the antioxidant mechanisms induced by Cr(6+) and selecting the organic-specific sensitive biomarkers to monitor the safety of the aquatic ecosystem.
六价铬（Cr(6+)）是环境中一种常见的污染物，具有较高的毒性。其毒性作用部分源于 Cr(6+)还原过程中活性氧（ROS）产生过多而导致的氧化损伤。为了研究 ROS 对氧化应激产生和抗氧化系统的直接影响，本研究首先从彭泽鲫中成功获得了抗氧化相关基因 cat、gpx1 和 Cu/Zn-sod 的全长 cDNA，并进行了分析。此外，还研究了急性暴露于 0.1、1.0、10 和 100mg/L Cr(6+)4 天后，彭泽鲫（Carassius auratus var. Pengze）幼鱼鳃、肠和肝中抗氧化基因（包括 cat、Cu/Zn-sod 和 gpx1）的 mRNA 表达、抗氧化酶活性（CAT、SOD 和 GPx）和总蛋白含量的变化。在不同组织中观察到抗氧化酶和基因表达的显著差异变化。这些发现有助于更好地理解 Cr(6+)诱导的抗氧化机制，并选择有机特异性敏感生物标志物来监测水生态系统的安全性。
