Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, Gwangju, Korea.
J Biomed Mater Res B Appl Biomater. 2010 May;93(2):484-91. doi: 10.1002/jbm.b.31606.
Implant failure has been attributed to loosening of an implant from the host bone possibly due to poor osseointegration. One promising strategy for improving osseointegration is to develop a functional implant surface that promotes osteoblast differentiation. In this study, a titanium (Ti) surface was functionalized by an anodic oxidation process and was loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2). The following four groups of Ti surfaces were prepared: machined (M), anodized machined (MA), resorbable blast medium (RBM), and anodized RBM (RBMA). The surfaces were characterized by scanning electron microscopy and contact angle measurements. The results showed that a Ti oxide layer (TiO(2)) was observed in the anodized surfaces in the form of nanotubes, approximately 100 nm in diameter and 500 nm in length. The hydrophilic properties of the anodized surfaces were significantly improved. The adsorbed rhBMP-2 loaded on the nonanodized surfaces and lyophilized showed spherical particle morphology. However, the adsorbed rhBMP-2 showed a dispersed pattern over the anodized surfaces. The velocity of the rhBMP-2 released from the surfaces was measured to determine if the anodized surface can improve in delivery efficiency. The results showed that the release velocity of the rhBMP-2 from the anodized surfaces was sustained when compared with that of the nonanodized surfaces. In addition, the rhBMP-2 released from the surface was found to be bioactive according to the alkaline phosphatase activity and the level of calcium mineral deposition. These results suggest that the TiO(2) nanotubular structure formed by anodizing is a promising configuration for sustained rhBMP-2 delivery.
种植体失败的原因可能是由于种植体与宿主骨之间的松动，这可能是由于骨整合不良所致。改善骨整合的一种有前途的策略是开发一种促进成骨细胞分化的功能性种植体表面。在这项研究中，通过阳极氧化工艺对钛（Ti）表面进行了功能化，并负载了重组人骨形态发生蛋白-2（rhBMP-2）。制备了以下四组 Ti 表面：机械加工（M）、阳极氧化机械加工（MA）、可吸收爆炸介质（RBM）和阳极氧化 RBM（RBMA）。通过扫描电子显微镜和接触角测量对表面进行了表征。结果表明，在阳极氧化表面观察到 Ti 氧化物层（TiO(2)）呈纳米管形式，直径约为 100nm，长度约为 500nm。阳极氧化表面的亲水性得到了显著改善。负载在非阳极氧化表面上并冻干的吸附 rhBMP-2 呈球形颗粒形态。然而，吸附在阳极氧化表面上的 rhBMP-2 呈现出分散的图案。测量了从表面释放的 rhBMP-2 的速度，以确定阳极氧化表面是否可以提高递送效率。结果表明，与非阳极氧化表面相比，从阳极氧化表面释放 rhBMP-2 的速度是持续的。此外，根据碱性磷酸酶活性和钙矿物质沉积水平，发现从表面释放的 rhBMP-2 具有生物活性。这些结果表明，阳极氧化形成的 TiO(2)纳米管结构是持续递送 rhBMP-2 的有前途的结构。
