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骨形态发生蛋白-2与纳米羟基磷灰石联合应用对牙种植体骨结合的影响

Effects of the combination of bone morphogenetic protein-2 and nano-hydroxyapatite on the osseointegration of dental implants.

作者信息

Pang KangMi, Seo Young-Kwon, Lee Jong-Ho

机构信息

Department of Dentistry, Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea.

Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul, Korea.

出版信息

J Korean Assoc Oral Maxillofac Surg. 2021 Dec 31;47(6):454-464. doi: 10.5125/jkaoms.2021.47.6.454.

DOI:10.5125/jkaoms.2021.47.6.454
PMID:34969019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8721409/
Abstract

OBJECTIVES

This study aimed to investigate the osteoinductivity of the combination of bone morphogenetic protein-2 (BMP-2) and nanohydroxyapatite (nHAp) and the effects of implants coated with nHAp/BMP-2.

MATERIALS AND METHODS

To evaluate the efficacy of nHAp/BMP-2 on bone formation, bone marrow-derived mesenchymal stem cells (BMMSCs) were seeded onto titanium disks coated with collagen (Col), Col/nHAp, or Col/nHAp/BMP-2. Protein levels were determined by a biochemical assay and reverse transcriptase-polymerase chain reaction. Stem cell differentiation was analyzed by flow cytometry. For studies with mice, Col, Col/nHAp, and Col/nHAp/BMP-2 were injected in subcutaneous pockets. Titanium implants or implants coated with Col/nHAp/BMP-2 were placed bilaterally on rabbit tibias and evaluated for 4 weeks.

RESULTS

In the study, BM-MSCs on Col/nHAp/BMP-2 showed reduced levels of CD73, CD90, and CD105 and increased levels of glycosaminoglycan, osteopontin, and alkaline phosphatase activity. After 4 weeks, the Col/nHAp/BMP-2 implant showed greater bone formation than the control (=0.07), while no differences were observed in bone implant contact and removal torque.

CONCLUSION

These results suggest that a combination of BMP-2 and an nHAp carrier would activate osseointegration on dental implant surfaces.

摘要

目的

本研究旨在探究骨形态发生蛋白-2（BMP-2）与纳米羟基磷灰石（nHAp）组合的骨诱导活性以及涂覆有nHAp/BMP-2的植入物的效果。

材料与方法

为评估nHAp/BMP-2对骨形成的功效，将骨髓间充质干细胞（BMMSCs）接种到涂有胶原蛋白（Col）、Col/nHAp或Col/nHAp/BMP-2的钛盘上。通过生化分析和逆转录聚合酶链反应测定蛋白质水平。通过流式细胞术分析干细胞分化。对于小鼠研究，将Col、Col/nHAp和Col/nHAp/BMP-2注射到皮下囊袋中。将钛植入物或涂有Col/nHAp/BMP-2的植入物双侧放置在兔胫骨上并评估4周。

结果

在该研究中，Col/nHAp/BMP-2上的BM-MSCs显示CD73、CD90和CD105水平降低，糖胺聚糖、骨桥蛋白水平和碱性磷酸酶活性增加。4周后，Col/nHAp/BMP-2植入物显示出比对照组更大的骨形成（P=0.07），而在骨植入物接触和去除扭矩方面未观察到差异。

结论

这些结果表明，BMP-2与nHAp载体的组合将激活牙种植体表面的骨整合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/2f2ab54be2b7/jkaoms-47-6-454-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/e4ef5533b312/jkaoms-47-6-454-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/3a3851fbf34b/jkaoms-47-6-454-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/4d01514fa957/jkaoms-47-6-454-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/6b86fdabb089/jkaoms-47-6-454-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/c1d13bcac899/jkaoms-47-6-454-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/2f2ab54be2b7/jkaoms-47-6-454-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/e4ef5533b312/jkaoms-47-6-454-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/3a3851fbf34b/jkaoms-47-6-454-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/4d01514fa957/jkaoms-47-6-454-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/6b86fdabb089/jkaoms-47-6-454-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/c1d13bcac899/jkaoms-47-6-454-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e9f/8721409/2f2ab54be2b7/jkaoms-47-6-454-f6.jpg

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