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纳米多孔不锈钢材料的细胞反应及药物递送效能评估

Evaluation of cellular response and drug delivery efficacy of nanoporous stainless steel material.

作者信息

Bae Inho, Lim Kyung-Seob, Park Jun-Kyu, Song Ju Han, Oh Sin-Hye, Kim Jung-Woo, Zhang Zijiao, Park Chan, Koh Jeong-Tae

机构信息

Hard-tissue Biointerface Research Center; Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju, 61186, Republic of Korea.

National Primate Research Center & Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, 28116, Republic of Korea.

出版信息

Biomater Res. 2021 Sep 26;25(1):30. doi: 10.1186/s40824-021-00232-8.

DOI:10.1186/s40824-021-00232-8
PMID:34565474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8474832/
Abstract

OBJECTIVE

Various surface modification techniques that can further improve the function and usability of stainless steel as a medical device have been reported. In the present study, the physical and biological properties of nanoporous stainless steel as well as its usefulness for drug delivery were assessed.

METHODS

The specimen was prepared with a circular disk shape (15 mm in diameter and 1 mm in thickness). The disk was subjected to electropolishing at a constant voltage of 20 V and 10 A for 10 min in an acidic environment (50% HSO). Everolimus (EVL) was used as a testing drug for drug-loading capacity of the material surface and release kinetics. The physiobiological properties of the material were assessed using platelet adhesion, and smooth muscle cell (SMC) adhesion, migration, and proliferation assays.

RESULTS

The surface roughness of the postpolishing group was greater than that of the nonpolishing group. Platelet adhesion and SMC adhesion and migration were inhibited in the postpolishing group compared to those in the prepolishing group. In the postpolishing group, the total amount of EVL on the surface (i.e., drug storage rate) was higher and the drug release rate was lower, with half the amount of the EVL released within 4 days compared with only 1 day for that of the prepolishing group.

CONCLUSION

Taken together, this stainless steel with a nanoporous surface could be used as a medical device for controlling cellular responses and carrying drugs.

摘要

目的

已有报道称多种表面改性技术可进一步提升不锈钢作为医疗器械的功能和实用性。在本研究中，对纳米多孔不锈钢的物理和生物学特性及其在药物递送方面的用途进行了评估。

方法

将样本制备成圆盘形状（直径15毫米，厚度1毫米）。在酸性环境（50%硫酸）中，圆盘在20伏恒定电压和10安电流下进行10分钟的电解抛光。依维莫司（EVL）用作测试药物，以评估材料表面的载药量和释放动力学。使用血小板黏附以及平滑肌细胞（SMC）黏附、迁移和增殖试验来评估材料的生理生物学特性。

结果

抛光后组的表面粗糙度大于未抛光组。与抛光前组相比，抛光后组的血小板黏附以及SMC黏附与迁移受到抑制。在抛光后组中，表面上EVL的总量（即药物储存率）更高，药物释放率更低，与抛光前组相比，EVL在4天内释放量为一半，而抛光前组仅在1天内释放一半。

结论

综上所述，这种具有纳米多孔表面的不锈钢可作为一种用于控制细胞反应和携带药物的医疗器械。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/2ceab9989a88/40824_2021_232_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/b45838a8141a/40824_2021_232_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/504bf214cef8/40824_2021_232_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/9e92674e9483/40824_2021_232_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/7ac27b6372af/40824_2021_232_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/d40122e6b2c6/40824_2021_232_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/7b29077b65d7/40824_2021_232_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/aa4bc5346df0/40824_2021_232_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/2ceab9989a88/40824_2021_232_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/b45838a8141a/40824_2021_232_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/504bf214cef8/40824_2021_232_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/9e92674e9483/40824_2021_232_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/7ac27b6372af/40824_2021_232_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/d40122e6b2c6/40824_2021_232_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/7b29077b65d7/40824_2021_232_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/aa4bc5346df0/40824_2021_232_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b87/8474832/2ceab9989a88/40824_2021_232_Fig8_HTML.jpg

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