Dept. of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
Biotechnol Prog. 2010 May-Jun;26(3):679-86. doi: 10.1002/btpr.381.
The autotrophic growth of an oil-rich indigenous microalgal isolate, identified as Chlorella vulgaris C--C, was promoted by using engineering strategies to obtain the microalgal oil for biodiesel synthesis. Illumination with a light/dark cycle of 14/10 (i.e., 14 h light-on and 10 h light-off) resulted in a high overall oil production rate (v(oil)) of 9.78 mg/L/day and a high electricity conversion efficiency (E(c)) of 23.7 mg cell/kw h. When using a NaHCO(3) concentration of 1,500 mg/L as carbon source, the v(oil) and E(c) were maximal at 100 mg/L/day and 128 mg/kw h, respectively. A Monod type model was used to describe the microalgal growth kinetics with an estimated maximum specific growth rate (mu(max)) of 0.605 day(-1) and a half saturation coefficient (K(s)) of 124.9 mg/L. An optimal nitrogen source (KNO(3)) concentration of 625 mg/L could further enhance the microalgal biomass and oil production, leading to a nearly 6.19 fold increase in v(oil) value.
利用工程策略促进富含油的土著微藻分离物（鉴定为普通小球藻 C--C）的自养生长，以获得用于生物柴油合成的微藻油。采用 14/10（即 14 小时光照和 10 小时黑暗）的光照/黑暗周期，导致总产油率（v(oil)）高达 9.78 mg/L/天和高的电能转换效率（E(c)）为 23.7 mg 细胞/千瓦小时。当使用 1500 mg/L 的 NaHCO(3)作为碳源时，v(oil)和 E(c)分别在 100 mg/L/天和 128 mg/千瓦小时达到最大值。使用 Monod 型模型来描述微藻的生长动力学，估计最大比生长速率（μ(max)）为 0.605 天(-1)，半饱和系数（K(s)）为 124.9 mg/L。最佳氮源（KNO(3)）浓度为 625 mg/L 可以进一步提高微藻的生物量和产油量，导致 v(oil)值增加近 6.19 倍。
