IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Bioresour Technol. 2012 Aug;118:61-6. doi: 10.1016/j.biortech.2012.05.055. Epub 2012 May 23.
Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic conditions. Moreover, supplementation of the inorganic culture medium with hydrolyzed cheese whey powder solution led to a significant improvement in microalgal biomass production and carbohydrate utilization when compared with the culture enriched with a mixture of pure glucose and galactose, due to the presence of growth promoting nutrients in cheese whey. Mixotrophic cultivation of C. vulgaris using the main dairy industry by-product could be considered a feasible alternative to reduce the costs of microalgal biomass production, since it does not require the addition of expensive carbohydrates to the culture medium.
研究了不同混养条件下小球藻的生长参数和生化成分，并将其与光自养对照培养物进行了比较。与光自养培养物相比，混养微藻具有更高的比生长速率、最终生物量浓度和脂质、淀粉和蛋白质的生产力。此外，与用纯葡萄糖和半乳糖混合物富集的培养基相比，用水解奶酪乳清粉溶液补充无机培养基可显著提高微藻生物量的产生和碳水化合物的利用，因为奶酪乳清中存在促进生长的营养物质。利用主要乳制品工业副产物进行小球藻的混养培养，可以考虑作为降低微藻生物量生产成本的可行替代方案，因为它不需要向培养基中添加昂贵的碳水化合物。
