Chen Pei-zhen, Wang Li-gang, Wang Ying-chun, Li Ji, Ding Wei, Ren Tian-zhi, Li Shao-peng
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Huan Jing Ke Xue. 2009 Dec;30(12):3614-8.
A heterotrophic nitrification-aerobic denitrifier bacteria CPZ24 was isolated form the livestock wastewater by way of the limiting dilution combined with the chromogenic medium screening methods. This bacterium was Gram positive, rod. The colonies of the strain were orange-red.It was identified as Rhodococuus pyridinivorans according to its morphological and physiological properties and the analysis of its 16S rDNA gene. Studied on its function of heterotrophic nitrification and aerobic denitrification,the results show that all NH4+ -N is removed and the removal rate of TN is 98.70% in heterotrophic nitrification; the removal rate of NO3- -N by this strain is 66.74% and the removal rate of TN is 64.27%. This high effective microorganisms with nitrogen removed is able to realize simultaneous nitrification and denitrification. It can perform the whole process of bacteria denitrification independently.
通过有限稀释结合显色培养基筛选法，从畜禽废水中分离出一株异养硝化-好氧反硝化细菌CPZ24。该菌为革兰氏阳性菌，呈杆状。菌株菌落为橙红色。根据其形态和生理特性以及16S rDNA基因分析，鉴定为嗜吡啶红球菌。对其异养硝化和好氧反硝化功能进行研究，结果表明，在异养硝化过程中，NH4+-N全部被去除，TN去除率为98.70%；该菌株对NO3--N的去除率为66.74%，TN去除率为64.27%。这种高效的脱氮微生物能够实现同步硝化反硝化，可独立完成细菌反硝化的全过程。
