School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea.
Biochem Biophys Res Commun. 2010 Mar 26;394(1):170-2. doi: 10.1016/j.bbrc.2010.02.138. Epub 2010 Feb 24.
Melittin is a well-known antimicrobial peptide with membrane-active mechanisms. In this study, it was found that Melittin exerted its antifungal effect via apoptosis. Candida albicans exposed to Melittin showed the increased reactive oxygen species (ROS) production, measured by DHR-123 staining. Fluorescence microscopy staining with FITC-annexin V, TUNEL and DAPI further confirmed diagnostic markers of yeast apoptosis including phosphatidylserine externalization, and DNA and nuclear fragmentation. The current study suggests that Melittin possesses an antifungal effect with another mechanism promoting apoptosis.
蜂毒素是一种具有膜活性机制的知名抗菌肽。在这项研究中发现，蜂毒素通过细胞凋亡发挥其抗真菌作用。通过 DHR-123 染色测量，发现暴露于蜂毒素的白色念珠菌表现出增加的活性氧 (ROS) 产生。用 FITC-annexin V、TUNEL 和 DAPI 进行荧光显微镜染色进一步证实了酵母凋亡的诊断标记物，包括磷脂酰丝氨酸外翻和 DNA 及核片段化。本研究表明，蜂毒素具有另一种促进细胞凋亡的抗真菌作用机制。
