School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Republic of Korea.
Biochem Biophys Res Commun. 2009 Dec 25;390(4):1255-9. doi: 10.1016/j.bbrc.2009.10.131. Epub 2009 Oct 28.
The antifungal effects and mechanisms of Styraxjaponoside C were investigated. Styraxjaponoside C was active against several human pathogens, including Candida albicans. Styraxjaponoside C induced a series of cellular changes characteristic of apoptosis in C. albicans, including increased reactive oxygen species (ROS) production, measured by DHR-123 staining; phosphatidylserine externalization, visualized by Annexin V staining; DNA fragmentation, as seen by TUNEL; and plasma membrane depolarization, observed by DiBAC(4)(3) staining. The plasma membrane depolarization is likely to be associated with production of ROS. The current study suggests that Styraxjaponoside C exerts an antifungal effect by promoting apoptosis.
研究了 Styraxjaponoside C 的抗真菌作用和机制。 Styraxjaponoside C 对包括白色念珠菌在内的几种人类病原体具有活性。 Styraxjaponoside C 诱导白色念珠菌发生一系列典型的细胞凋亡变化，包括通过 DHR-123 染色检测到的活性氧（ROS）产生增加；通过 Annexin V 染色观察到的磷脂酰丝氨酸外翻；通过 TUNEL 观察到的 DNA 片段化；以及通过 DiBAC(4)(3)染色观察到的质膜去极化。这种质膜去极化可能与 ROS 的产生有关。本研究表明， Styraxjaponoside C 通过促进细胞凋亡发挥抗真菌作用。
