Peng Cui, Liu Yang, Shui Liangyong, Zhao Zhongyi, Mao Xinfang, Liu Zhongyuan
College of Chemical Engineering, Sichuan University of Science and Engineering, 180 Xueyuan Street, Zigong 643000, China.
Life (Basel). 2022 Oct 11;12(10):1581. doi: 10.3390/life12101581.
The development of drug resistance has caused fungal infections to become a global health concern. Antimicrobial peptides (AMPs) offer a viable solution to these pathogens due to their resistance to drug resistance and their diverse mechanisms of actions, which include direct killing and immunomodulatory properties. The peptide Cecropin, which is expressed by genetically engineered bacteria, has antifungal effects on . The minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) of were 0.9 μg/mL and 1.8 μg/mL, respectively, detected by the micro-broth dilution method. According to the killing kinetics, the MFC of Cecropin could kill in 40 min. The electron microscope indicated that Cecropin could cause the cell wall to become rough and nicked, eventually killing . The effects of Cecropin on the cell membrane of treated , using the 1,6-diphenyl-1,3,5-hexatriene and propidium iodide protocol, showed that they could change the permeability and fluidity, destroy it, and lead to cell necrosis. In addition, Cecropin can also induce cells to produce excessive reactive oxygen species, causing changes in the mitochondrial membrane potential. Therefore, this study provides a certain theoretical basis for the antifungal infection of new antifungal agents.
耐药性的发展已使真菌感染成为全球卫生关注的问题。抗菌肽（AMPs）因其对耐药性具有抗性且作用机制多样，包括直接杀伤和免疫调节特性，为应对这些病原体提供了一种可行的解决方案。通过基因工程细菌表达的天蚕素肽对……具有抗真菌作用。采用微量肉汤稀释法检测，其最小抑菌浓度（MIC）和最小杀菌浓度（MFC）分别为0.9μg/mL和1.8μg/mL。根据杀菌动力学，天蚕素肽的MFC可在40分钟内杀死……。电子显微镜显示，天蚕素肽可导致细胞壁变得粗糙且有缺口，最终杀死……。使用1,6 - 二苯基 - 1,3,5 - 己三烯和碘化丙啶方法，研究天蚕素肽对经处理的……细胞膜的影响，结果表明其可改变通透性和流动性，破坏细胞膜并导致细胞坏死。此外，天蚕素肽还可诱导细胞产生过量活性氧，引起线粒体膜电位变化。因此，本研究为新型抗真菌药物的抗真菌感染提供了一定的理论依据。
