Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Clin Chim Acta. 2010 May 2;411(9-10):765-70. doi: 10.1016/j.cca.2010.02.063. Epub 2010 Feb 24.
Bioactive lipid mediator S1P has been suggested to play pathophysiological roles in various fields of clinical science as a circulating paracrine mediator. We previously established a reliable method of measuring plasma S1P concentration, and reported that the one in healthy subjects has a gender difference and a correlation with red blood cell (RBC)-parameters, however, the reports of S1P measurements in the blood in patients with a specific disease have been scarce. Because our previous evidence suggests that S1P is involved in liver pathophysiology, we examined plasma S1P concentration in chronic hepatitis C patients.
S1P assay was performed using a high-performance liquid chromatography system.
Plasma S1P concentrations were reduced in chronic hepatitis C patients compared with in healthy subjects with the same hemoglobin concentration, irrespective of gender. Among the blood parameters, serum hyaluronic acid concentration, a surrogate marker for liver fibrosis, was most closely and inversely correlated with plasma S1P concentration. Furthermore, plasma S1P concentration decreased throughout the progression of carbon tetrachloride-induced liver fibrosis in rats.
Plasma S1P concentration was reduced in chronic hepatitis C patients, and liver fibrosis might be involved, at least in part, in the mechanism responsible for this reduction.
作为一种循环旁分泌介质，生物活性脂质介质 S1P 被认为在临床科学的各个领域中发挥着病理生理作用。我们之前建立了一种可靠的测量血浆 S1P 浓度的方法，并报告了健康受试者的 S1P 浓度存在性别差异，且与红细胞（RBC）参数相关，然而，关于特定疾病患者血液中 S1P 测量的报告却很少。因为我们之前的证据表明 S1P 参与了肝脏的病理生理学，所以我们检查了慢性丙型肝炎患者的血浆 S1P 浓度。
使用高效液相色谱系统进行 S1P 测定。
与具有相同血红蛋白浓度的健康受试者相比，慢性丙型肝炎患者的血浆 S1P 浓度降低，而与性别无关。在血液参数中，血清透明质酸浓度（肝纤维化的替代标志物）与血浆 S1P 浓度的相关性最密切且呈负相关。此外，在四氯化碳诱导的大鼠肝纤维化进展过程中，血浆 S1P 浓度逐渐降低。
慢性丙型肝炎患者的血浆 S1P 浓度降低，肝纤维化可能至少部分参与了导致这种降低的机制。
