Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
J Biotechnol. 2010 May 3;147(1):59-63. doi: 10.1016/j.jbiotec.2010.02.013. Epub 2010 Feb 24.
An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.
一种抗氧化脂质体催化剂 LIPOzyme，通过使用简单配体（十二酰组氨酸，Dodec-His）和金属离子（Mn）修饰的脂质体模拟超氧化物歧化酶（SOD）和过氧化物酶（POD）样活性。SOD 样活性取决于脂质体膜上配体-金属配合物的稳定性，DPPC 脂质体的稳定性更高，pH 值更高。发现 DMPC 脂质体的过氧化物酶样活性最大，其中配体-金属配合物更深入地插入到膜中。因此，已经表明用简单配体修饰的脂质体可以根据脂质体膜的特性表现出不同的酶样活性。
