半胱氨酸偶联的钆-DTPA 修饰的金纳米粒子作为多模态生物成像剂。

Gold nanoparticles functionalized by gadolinium-DTPA conjugate of cysteine as a multimodal bioimaging agent.

机构信息

Laboratory of Nuclear Medicine Research, Molecular Imaging Center, Korea Institute of Radiological and Medical Science, Seoul, Republic of Korea.

出版信息

Bioorg Med Chem Lett. 2010 Apr 1;20(7):2287-91. doi: 10.1016/j.bmcl.2010.02.002. Epub 2010 Feb 6.

DOI:10.1016/j.bmcl.2010.02.002

PMID:20188545

Abstract

The synthesis and characterization of gold nanoparticles coated with Gd-chelate (Au@GdL), where L is a conjugate of DTPA and cysteine, is described. These particles are obtained by the replacement of citrate from the gold nanoparticle surfaces with gadolinium chelate (GdL). The average size of Au@GdL is 14 nm with a loading of GdL reaching up to 2.9x10(3) per particles, and they demonstrate very high R1 relaxivity (approximately 10(5) mM(-1) s(-1)) as well as X-ray attenuation. The R1 relaxivity per [Gd] is 17.9 mM(-1) s(-1). The present system also exhibits macrophage-specific property, as demonstrated by histological and TEM images as well as CT and MR, rendering itself as a new class of T1 multimodal CT/MR contrast agent.

摘要

本文描述了一种金纳米粒子表面包覆 Gd-螯合物（Au@GdL）的合成与表征，其中 L 是 DTPA 和半胱氨酸的共轭物。这些粒子是通过用 Gd-螯合物（GdL）取代金纳米粒子表面的柠檬酸根而得到的。Au@GdL 的平均粒径为 14nm，GdL 的载量高达每个粒子 2.9x10(3)，其 R1 弛豫率非常高（约 10(5) mM(-1) s(-1))，同时具有 X 射线衰减能力。每 [Gd] 的 R1 弛豫率为 17.9 mM(-1) s(-1)。本系统还表现出巨噬细胞特异性，这一点可以通过组织学和 TEM 图像以及 CT 和 MR 得到证明，使其成为一类新的 T1 多模态 CT/MR 对比剂。