Allen T D, Testa N G
Department of Ultrastructure, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Blood Cells. 1991;17(1):29-38; discussion 39-43.
Long-term bone marrow cultures (LTBMC) are readily converted from the usual granulopoietic to erythropoietic production by the addition of anemic mouse serum (AMS). The "statics" of proliferation and maturation, previously shown by ultrastructural methods to closely mirror the in vivo situation, were studied dynamically using a time-lapse video system. Several cell pedigrees were followed, but the most complete series showed three successive divisions and subsequent enucleations in the progeny of three synchronously mitotic cells observed in the culture; this is indicative of a five division sequence in the erythron. As in erythroblastic islets observed in marrow in vivo, the striking synchrony of maturation was maintained in vitro. Furthermore, when some of the erythroid progeny became displaced to other macrophages, the synchrony, which was maintained by the original erythroid group on the original erythroblastic islet macrophage, was lost. Time-lapse video, which is inexpensive to run and can be maintained in continuous recording for many weeks, is an ideal technique for recording both erythroid cell pedigrees, and the initial events leading to the formation of an erythroblastic islet in vitro after stimulation with AMS.
长期骨髓培养（LTBMC）通过添加贫血小鼠血清（AMS）可很容易地从通常的粒细胞生成转变为红细胞生成。先前通过超微结构方法显示增殖和成熟的“静态”情况能紧密反映体内情况，本研究使用延时视频系统对其进行了动态研究。追踪了几个细胞谱系，但最完整的系列显示在培养物中观察到的三个同步有丝分裂细胞的后代中有三次连续分裂及随后的去核过程；这表明红细胞系存在一个五分裂序列。与在体内骨髓中观察到的成红细胞岛一样，成熟的显著同步性在体外得以维持。此外，当一些红系后代转移到其他巨噬细胞时，由原始成红细胞岛巨噬细胞上的原始红系群体维持的同步性丧失。延时视频运行成本低廉，且可连续记录数周，是记录红系细胞谱系以及在用AMS刺激后体外形成成红细胞岛的初始事件的理想技术。
