Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Kilómetro 0.6 carretera la Victoria, Hermosillo 83304, Sonora, Mexico.
Viruses. 2019 Oct 31;11(11):1001. doi: 10.3390/v11111001.
Conventional dendritic cells (cDCs) cannot be infected by porcine reproductive and respiratory syndrome virus (PRRSV) but respond to infection via cytokine production, indicating a possible role in initiation/regulation of the immune response against PRRSV. In this work, we evaluated the responses of splenic and blood cDCs, with DEC205CADM1CD172a phenotype, as well as those of CD163 cells against PRRSV and porcine epidemic diarrhea virus (PEDV). Both populations were incubated in the presence of PRRSV or PEDV with and without naïve CD3 T cells, and cytokine responses were evaluated by qPCR and ELISA. Our results showed that cDCs, but not CD163 cells, produced IL-12 in response to PRRSV. PEDV did not induce IL-12 production. Cocultures of cDCs and autologous naïve CD3 cells resulted in decreased IL-12 production and low expression of IFN-γ transcripts in response to PRRSV. Interestingly, cDCs increased the proliferation of naïve T cells in the presence of PRRSV compared with that achieved with monocytes and peripheral blood mononuclear cells (PBMCs). Cocultures of CD163 cells induced IL-10 and IL-4 expression in the presence of PRRSV and PEDV, respectively. In conclusion, cDCs can selectively produce IL-12 in response to PRRSV but poorly participate in the activation of naïve T cells.
传统树突状细胞（cDCs）不能被猪繁殖与呼吸综合征病毒（PRRSV）感染，但可通过细胞因子产生来响应感染，这表明其在 PRRSV 免疫反应的启动/调节中可能发挥作用。在这项工作中，我们评估了具有 DEC205CADM1CD172a 表型的脾和血 cDCs 以及 CD163 细胞对 PRRSV 和猪流行性腹泻病毒（PEDV）的反应。将这两种细胞群在存在 PRRSV 或 PEDV 的情况下与和不与幼稚 CD3 T 细胞孵育，并通过 qPCR 和 ELISA 评估细胞因子反应。我们的结果表明，cDCs 而不是 CD163 细胞会对 PRRSV 产生 IL-12。PEDV 不会诱导 IL-12 的产生。cDCs 与自体幼稚 CD3 细胞共培养会导致对 PRRSV 的 IL-12 产生减少和 IFN-γ 转录物的低表达。有趣的是，与单核细胞和外周血单核细胞（PBMCs）相比，cDCs 在存在 PRRSV 的情况下会增加幼稚 T 细胞的增殖。CD163 细胞的共培养会在存在 PRRSV 和 PEDV 的情况下分别诱导 IL-10 和 IL-4 的表达。总之，cDCs 可以选择性地对 PRRSV 产生 IL-12，但对幼稚 T 细胞的激活参与较少。
