Department of Physiology, Faculty of Sciences, University of Extremadura, 06071 Badajoz, Spain.
Brain Behav Immun. 2010 May;24(4):672-7. doi: 10.1016/j.bbi.2010.02.003. Epub 2010 Feb 25.
The blood concentration of extracellular 72kDa heat shock protein (eHsp72) increases under conditions of stress, including intense exercise. However, the signal(s), source(s), and secretory pathways in its release into the bloodstream have yet to be clarified. The aim of the present study was to evaluate the role of noradrenaline (NA) as a stress signal on the expression and release of Hsp72 by circulating neutrophils (as a source), all within a context of the immunophysiological regulation during exercise-induced stress in sedentary and healthy young (21-26years) women. The expression of Hsp72 on the surface of isolated neutrophils was determined by flow cytometry, and its release by cultured isolated neutrophils was determined by ELISA. Incubation with cmHsp70-FITC showed that neutrophils express Hsp72 on their surface under basal conditions. In addition, cultured isolated neutrophils (37 degrees C and 5% CO(2)) also released Hsp72 under basal conditions, with this release increasing from 10min to 24h in the absence of cell damage. NA at 10(-9)-10(-5)M doubled the percentage of neutrophils expressing Hsp72 after 60min and 24h incubation. NA also stimulated (by about 20%) the release of Hsp72 after 10min of incubation.
(1) Hsp72 is expressed on the surface of isolated neutrophils under basal conditions, and this expression is augmented by NA. (2) Isolated neutrophils can also release Hsp72 under cultured basal conditions in the absence of cell death, and NA can increase this release. These results may contribute to confirming the hypothesis that NA can act as a "stress signal" for the increased eHsp72 in the context of exercise stress, with a role for neutrophils as a source for the expression and, to a lesser degree, the release of Hsp72 after activation by NA.
细胞外 72kDa 热休克蛋白（eHsp72）的血液浓度在应激条件下增加，包括剧烈运动。然而，其释放到血液中的信号、来源和分泌途径尚未阐明。本研究的目的是评估去甲肾上腺素（NA）作为应激信号在运动应激过程中免疫生理调节下循环中性粒细胞（作为来源）中 HSP72 的表达和释放中的作用。通过流式细胞术测定分离的中性粒细胞表面 HSP72 的表达，通过 ELISA 测定培养分离的中性粒细胞的释放。孵育 cmHsp70-FITC 表明，在基础条件下，中性粒细胞在其表面表达 HSP72。此外，培养的分离中性粒细胞（37°C 和 5%CO2）在基础条件下也释放 HSP72，在没有细胞损伤的情况下，这种释放从 10min 增加到 24h。10-9-10-5M 的 NA 在孵育 60min 和 24h 后使表达 HSP72 的中性粒细胞百分比增加一倍。NA 还刺激（增加约 20%）孵育 10min 后的 HSP72 释放。
（1）在基础条件下，Hsp72 表达于分离的中性粒细胞表面，NA 增强这种表达。（2）在没有细胞死亡的情况下，培养的基础条件下，分离的中性粒细胞也可以释放 HSP72，NA 可以增加这种释放。这些结果可能有助于证实假说，即 NA 可以作为运动应激中 eHsp72 增加的“应激信号”，中性粒细胞作为表达的来源，并且在 NA 激活后，在较小程度上释放 HSP72。
