Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environmental Science and Engineering, Hohai University, 210098 Nanjing, China.
Chemosphere. 2010 Apr;79(5):588-94. doi: 10.1016/j.chemosphere.2010.01.053. Epub 2010 Feb 26.
Carassius auratus bred in clean water were selected to conduct active biomonitoring (ABM) exposures in Meiliang and Gong bays in the northern polluted area of Taihu Lake. The biotransformation enzymes 7-ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase (GST), antioxidant enzymes glutathione peroxidase (GP(X)), reduced glutathione (GSH) content and superoxide dismutase (SOD) and lipoperoxidation (as TBARS) in liver were determined as biomarkers during the field exposure period. At the same time, the contents of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and heavy metals in the surface sediments of biomonitoring sites were measured. The total PAH concentrations ranged from 248.8 to 375.9microgkg(-1) dry weight, PCB from 1.26 to 2.41microgkg(-1), OCP from 0.15 to 2.06microgkg(-1), and heavy metal from 126.3 to 210.0mgkg(-1). The results indicate that complex pollution by persistent organic pollutants and heavy metals is present in the northern end of Taihu Lake and the in situ exposed organisms were stressed. Moreover, Meilaing Bay is a more stressful place to fish living there. The integrated biomarker response (IBR) was calculated by combining different biomarkers to single value, which can be used to describe the toxically-induced stress level of populations in different areas. Pollutant concentrations were displayed as star plots and compared to IBR star plots. There was a visual correlation between the PCB gradient and the OCP gradient measured in the sediments and the IBR variation.
选择在清洁水中养殖的鲤鱼进行了太湖北部污染区梅梁湾和贡湖的主动生物监测（ABM）暴露实验。在野外暴露期间，测定了生物标志物肝脏中的 7-乙氧基香豆素-O-脱乙基酶（EROD）和谷胱甘肽 S-转移酶（GST）、抗氧化酶谷胱甘肽过氧化物酶（GP(X)）、还原型谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）和脂质过氧化（以 TBARS 表示）。同时，测定了监测点表层沉积物中多环芳烃（PAHs）、多氯联苯（PCBs）、有机氯农药（OCPs）和重金属的含量。总多环芳烃浓度范围为 248.8 至 375.9μgkg(-1)干重，PCBs 为 1.26 至 2.41μgkg(-1)，OCPs 为 0.15 至 2.06μgkg(-1)，重金属为 126.3 至 210.0mgkg(-1)。结果表明，太湖北部存在持久性有机污染物和重金属的复合污染，原位暴露的生物受到胁迫。此外，梅梁湾是一个对生活在那里的鱼类压力更大的地方。通过将不同的生物标志物组合成单一值来计算综合生物标志物反应（IBR），可以用来描述不同地区种群因毒物引起的胁迫水平。污染物浓度以星图显示，并与 IBR 星图进行比较。沉积物中测量的 PCB 梯度和 OCP 梯度与 IBR 变化之间存在明显的相关性。
