Borković-Mitić Slavica, Mitić Bojan, Vranković Jelena S, Jovičić Katarina, Pavlović Slađan
Department of Physiology, Institute for Biological Research "Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia.
Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski Trg 16, 11000 Belgrade, Serbia.
Toxics. 2024 Oct 18;12(10):756. doi: 10.3390/toxics12100756.
In this study, the activity of oxidative stress parameters superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione S-transferase (GST), as well as the concentrations of vitamin E (Vit E) and SH groups in the digestive glands and gills of freshwater mussels and from the Sava River in Serbia were investigated. These parameters were determined in native and invasive mussels under the same environmental conditions. The activities of GSH-Px and GR and the concentration of Vit E were significantly higher in the digestive glands of the autochthonous species than in the invasive species , while the CAT activity and the concentration of SH groups were lower. In the gills of , GSH-Px activity and Vit E concentration were significantly higher, while CAT, GST, and SH groups were lower. Principal component analysis (PCA) showed that oxidative stress parameters were strictly tissue- and species-specific. In addition, integrated biomarker response (IBR) showed a combined response of enzymatic and non-enzymatic oxidative stress parameters depending on the tissue or species studied, indicating different metabolic activities and behaviors of an autochthonous versus an introduced bivalve species.
在本研究中，对塞尔维亚萨瓦河淡水贻贝消化腺和鳃中氧化应激参数超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽还原酶（GR）和谷胱甘肽S-转移酶（GST）的活性，以及维生素E（Vit E）和SH基团的浓度进行了研究。在相同环境条件下，对本地贻贝和入侵贻贝的这些参数进行了测定。本地物种消化腺中GSH-Px和GR的活性以及Vit E的浓度显著高于入侵物种，而CAT活性和SH基团的浓度则较低。在鳃中，GSH-Px活性和Vit E浓度显著较高，而CAT、GST和SH基团较低。主成分分析（PCA）表明，氧化应激参数具有严格的组织特异性和物种特异性。此外，综合生物标志物反应（IBR）显示，酶促和非酶促氧化应激参数的综合反应取决于所研究的组织或物种，表明本地双壳类物种与引入双壳类物种的代谢活动和行为不同。
