Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Anand Nagar, Krishnankoil 626190, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):174-80. doi: 10.1016/j.colsurfb.2010.01.018. Epub 2010 Feb 4.
Biocompatible gold nanoparticles have received considerable attention in recent years because of their promising applications in bioimaging, biosensors, biolabels, and biomedicine. The generation of gold nanoparticles using extra-cellular alpha-amylase for the reduction of AuCl(4) with the retention of enzymatic activity in the complex is being reported. The enhanced synthesis of particles has been brought about by optimizing the medium components for alpha-amylase. Response surface methodology and central composite rotary design (CCRD) were employed to optimize a fermentation medium for the production of alpha-amylase by Bacillus licheniformis at pH 8. The three variables involved in the study of alpha-amylase were fructose, peptone and soya meal. Only fructose had a significant effect on alpha-amylase production. The most optimum medium (medB) containing (%) fructose: 3, peptone: 1, soya meal: 2, resulted in a amylase activity of 201.381 U/ml which is same as that of the central level. The least optimum (medA) and most optimum (medB) media were compared for the synthesis of particles indicated by difference in color formation. Spectrophotometric analysis revealed that the particles exhibited a peak at 582 nm and the A(582) for the Med B was 8-fold greater than that of the Med A. The TEM analysis revealed that the particle size ranged from 10 to 50 nm.
近年来，由于其在生物成像、生物传感器、生物标记和生物医学方面的应用前景广阔，生物相容性金纳米粒子受到了广泛关注。本文报道了一种使用细胞外α-淀粉酶还原 AuCl(4)并保持复合物中酶活性的方法来生成金纳米粒子。通过优化α-淀粉酶的介质成分，实现了颗粒的增强合成。响应面法和中心复合旋转设计（CCRD）被用于优化地衣芽孢杆菌生产α-淀粉酶的发酵培养基，在 pH 8 下进行。该研究中涉及的α-淀粉酶的三个变量是果糖、蛋白胨和豆粕。只有果糖对α-淀粉酶的生产有显著影响。最适培养基（medB）含有（%）果糖：3、蛋白胨：1、豆粕：2，酶活达到 201.381 U/ml，与中心点相同。对最适（medB）和最不适（medA）培养基进行了比较，以研究粒子的合成，这可以通过颜色形成的差异来表示。分光光度分析表明，粒子在 582nm 处有一个峰值，Med B 的 A(582)比 Med A 高 8 倍。TEM 分析表明，粒径范围为 10 至 50nm。
