深层发酵生产S2α-淀粉酶的优化。
Optimization of submerged S2 α-amylase production.
作者信息
Naili Belgacem, Sahnoun Mouna, Bejar Samir, Kammoun Radhouane
机构信息
Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, 3018 Tunisia.
出版信息
Food Sci Biotechnol. 2016 Feb 29;25(1):185-192. doi: 10.1007/s10068-016-0028-4. eCollection 2016.
Abstract
Use of 4 agro-industrial by products and organic materials as nitrogen sources for production of S2 α-amylase in liquid culture was investigated. The 2 agro-industrial byproducts maltose and saccharose, and also lactose and starch were individually evaluated for use as carbon sources. A Box-Behnken experimental design was used to determine optimal conditions for production of α-amylase. A maximum amylase activity of 750 U/mL was obtained at a temperature of 24°C, a urea concentration of 1 g/L, and a C/N ratio of 2. Laboratory scale application of optimal conditions in a 7 L fermentor produced a final α-amylase activity of 770 U/mL after 3 days of batch cultivation. Addition of 10% starch to the culture medium each 12 h immediately after the stationary phase of cell growth led to a production yield of 1,220 U/mL at the end of fed-batch cultivation.
摘要
研究了使用4种农业工业副产品和有机材料作为液体培养生产S2α-淀粉酶的氮源。对2种农业工业副产品麦芽糖和蔗糖以及乳糖和淀粉分别进行了作为碳源的评估。采用Box-Behnken实验设计来确定α-淀粉酶生产的最佳条件。在温度为24°C、尿素浓度为1 g/L和C/N比为2的条件下，获得了750 U/mL的最大淀粉酶活性。在7 L发酵罐中实验室规模应用最佳条件，分批培养3天后最终α-淀粉酶活性为770 U/mL。在细胞生长稳定期后每12 h向培养基中添加10%淀粉，补料分批培养结束时产量达到1220 U/mL。
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