Korea Ocean Research and Development Institute, Ansan, PO Box 29, Seoul 425-600, South Korea.
Curr Opin Biotechnol. 2010 Jun;21(3):353-7. doi: 10.1016/j.copbio.2010.01.015. Epub 2010 Feb 26.
The enormous pool of biodiversity in marine ecosystems is an excellent natural reservoir for acquiring an inventory of enzymes with potential for biotechnological applications. Moreover, the opportunity for sustainable resource management has been greatly enhanced by recent advances in culturing methods for recalcitrant microbes. In this review, we will focus primarily on successful examples in culturing marine microbes and provide an overview of work examining the biotechnological potential of the marine reservoir, mainly through genomic strategies, such as activity-based functional screening of genomic and metagenomic libraries and homology-driven screening of enormous amounts of sequence data.
海洋生态系统中丰富的生物多样性是获取具有生物技术应用潜力的酶类物质的绝佳天然资源库。此外，近年来，对顽固微生物培养方法的改进极大地促进了可持续资源管理的机会。在这篇综述中，我们将主要关注培养海洋微生物方面的成功案例，并概述通过基因组策略研究海洋资源库的生物技术潜力的工作，主要包括基于活性的基因组和宏基因组文库功能筛选以及大量序列数据的同源驱动筛选等方法。
