Microbial Genome Research Group, Extremobiosphere Research Program, Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan.
DNA Res. 2010 Jun;17(3):123-37. doi: 10.1093/dnares/dsq005. Epub 2010 Feb 26.
The complete genome sequence of the thermophilic sulphur-reducing bacterium, Deferribacter desulfuricans SMM1, isolated from a hydrothermal vent chimney has been determined. The genome comprises a single circular chromosome of 2,234,389 bp and a megaplasmid of 308,544 bp. Many genes encoded in the genome are most similar to the genes of sulphur- or sulphate-reducing bacterial species within Deltaproteobacteria. The reconstructed central metabolisms showed a heterotrophic lifestyle primarily driven by C1 to C3 organics, e.g. formate, acetate, and pyruvate, and also suggested that the inability of autotrophy via a reductive tricarboxylic acid cycle may be due to the lack of ATP-dependent citrate lyase. In addition, the genome encodes numerous genes for chemoreceptors, chemotaxis-like systems, and signal transduction machineries. These signalling networks may be linked to this bacterium's versatile energy metabolisms and may provide ecophysiological advantages for D. desulfuricans SSM1 thriving in the physically and chemically fluctuating environments near hydrothermal vents. This is the first genome sequence from the phylum Deferribacteres.
已完成从热液喷口烟囱中分离出的嗜热硫还原菌 Deferribacter desulfuricans SMM1 的全基因组测序。该基因组由一个 2,234,389 bp 的单环染色体和一个 308,544 bp 的大质粒组成。该基因组中许多编码的基因与δ变形菌中硫或硫酸盐还原细菌的基因最为相似。重建的中心代谢表明其异养生活方式主要由 C1 到 C3 有机物驱动，例如甲酸盐、乙酸盐和丙酮酸，并且还表明通过还原性三羧酸循环进行自养的能力可能是由于缺乏 ATP 依赖性柠檬酸裂解酶。此外，基因组编码了许多化学感受器、趋化样系统和信号转导机制的基因。这些信号网络可能与该细菌多样化的能量代谢有关，并为 D. desulfuricans SSM1 在热液喷口附近物理和化学变化的环境中生存提供生态生理优势。这是 Deferribacteres 门的第一个基因组序列。
